Zn^(2+)增强非洲爪蟾卵母细胞ATP-激活电流  

Augmentation of ATP-activated currents by Zn^(2+) in xenopus oocytes

作　　者：张玉芹[1] 程剑侠[1] 王照宇[1] 朱凡[1] 聂辉[1] 罗加烈[2] 李之望[2] 

机构地区：[1]武汉科技大学医学院生理学教研室,湖北武汉430065 [2]华中科技大学同济医学院神经生物学系,湖北武汉430030

出　　处：《基础医学与临床》2008年第1期18-21,共4页Basic and Clinical Medicine

基　　金：湖北省自然科学基金(2004ABA152);湖北省教育厅项目(2006B079)

摘　　要：目的研究Zn2+对非洲爪蟾卵母细胞ATP激活电流(IATP)的调制作用。方法用双电极电压钳技术记录不同浓度Zn2+对IATP的影响。结果Zn2+对IATP(尤其是D1部分)的影响有明显的浓度依赖性。3×10-5mol/L(30μmol/L)Zn2+可使IATP幅值增加(117.6±8.21)%,显著高于对照值(P<0.01);并使IATP量-效曲线左上移,但并不改变反应的最大幅值。结论Zn2+对ATP-激活电流调制效应可能是通过改变配体和受体的亲和力而实现的变构调制。Objective To study the modulatory effects of Zn^2＋ on ATP-activated currents （IATP ） of xenopus oocytes. Methods The two electrode-voltage clamp technique was used to record IATP in healthy xenopus oocytes by applying Zn^2＋ in extracellular fluid. Results We found that IATP（ especially the component D1 ） can be obviously modulated by Zn^2＋ in a concentration dependent manner. The amplitude of IATP be increased by （ 117.6 ± 8.21 ） % n = 11 ） when modulated by 3 × 10^ -5 mol/L Zn^2＋ , which showed a remarkable difference as compared with control value （P 〈0. 01 ）. Zn^2＋ can cause the concentration-response relationship curve towarding uP-left direction, while does not change the maximal amplitude. Conclusion The modulation effect of Zn^2＋ on IATP belongs to allosteric modulation and may be achieved by changing the affinity of ligand-receptor.

关 键 词：ZN^2+ ATP-激活电流 爪蟾卵母细胞 双电极电压钳技术 

分 类 号：R33[医药卫生—人体生理学]