血管生长素-1基因修饰骨髓间充质干细胞对黏附因子表达的影响  

Influence of angiopoietin-1 gene modified mesenchymal stem cells on the expression of adhension factors

作　　者：郭晋村[1] 王挹青[2] 谢良地[1] 许昌声[1] 

机构地区：[1]福建医科大学,福建福州350108 [2]厦门大学附属中山医院心内科,福建厦门361004

出　　处：《基础医学与临床》2008年第1期26-30,共5页Basic and Clinical Medicine

基　　金：卫生部科学研究基金(WKJ2005-2-011)

摘　　要：目的探讨血管生长素1(Ang1)基因转导的骨髓间充质干细胞(Ang1-rMSC)对内皮细胞黏附因子表达的影响。方法经慢病毒载体介导构建Ang1-rMSC,观察VEGF刺激下不同时间点人脐静脉血管内皮细胞(HUVEC)黏附分子VCAM-1和ICAM-1表达的动态改变。采用RT-PCR及Western blot技术检测黏附分子mRNA及蛋白质水平的表达变化。结果HUVEC在20μg/L VEGF刺激下,ICAM-1和VCAM-1 mRNA水平显著升高,8h达到峰值,与对照组相比分别增加4.2倍及3.2倍。不同浓度上清液孵育的HUVEC在VEGF刺激后,ICAM-1和VCAM-1 mRNA表达均较对照组下降;含70%Ang1-rMSC上清液孵育的HUVEC,VCAM-1与ICAM-1表达较单纯VEGF组显著下降。结论Ang1-rMSC上清液可抑制VEGF诱导的HUVEC黏附分子表达,通过Ang1基因修饰Ang1-rMSC用于抑制干细胞移植伴发的炎症反应具有可行性。Objective To investigate the influence of Angiopoietin-1 （ Angl ） gene modified mesenchymal stem cells on the expression of adhension factors from endothelial cells, to clarify the feasibility of modulating inflammation reaction by gene modification after stem cells transplantation. Methods Genetic engineering rMSCs were constructed by lentivirally-tranduced Angl gene, the expressions of ICAM-1 and VCAM-1 at different time points after 20μg/L VEGF stimulating were investigated, the superuatants of gene modified rMSC were collected and then add- ed into HUVEC culture with different concentrations of Angl, the expressions of adhension factors after VEGF stimulated were observed at the same time. Results Genetic engineering rMSCs were successfully constructed, the expressions of ICAM-1 and VCAM-1 mRNA were significantly increased after VEGF stimulated, the maximal value was at 8 h, the ICAM-1 and VCAM-1 mRNA expressions were decreased after incubation with Angl at different con- centrations, Angl suppressed the VEGF-stimulated protein levels of ICAM-1 and VCAM-1. Conclusion The superuatants of Angl gene modified stem cells can suppresse VEGF-stimulated inflammation reaction of HUVEC.

关 键 词：血管生长素-1 黏附分子 骨髓间充质干细胞 

分 类 号：R543.5[医药卫生—心血管疾病]