大孔吸附树脂分离纯化地黄中梓醇工艺的研究  

Study on the Separation and Purification Technology of Catalpol from Rehmannia by Macroporous Adsorption Resins

作　　者：张秀丽[1] 王静云[1] 姜波[1] 常妍[1] 安利佳[1] 

机构地区：[1]大连理工大学环境与生命学院生物化工系,大连116024

出　　处：《中国生物工程杂志》2008年第1期65-69,共5页China Biotechnology

基　　金：国际科技合作重点项目计划资助项目(2005DFA40790)

摘　　要：利用大孔吸附树脂分离提取地黄中梓醇。以地黄粗提液中梓醇含量为指标,高效液相色谱(HPLC)为含量测定方法,考察9种不同极性大孔吸附树脂对梓醇的吸附和解吸附性能,筛选出最佳树脂D101进行分离实验。结果表明,D101大孔吸附树脂的静态吸附容量为69.2mg/g干树脂,其吸附等温线符合Langmuir和Freundlich吸附等温式。采用5%乙醇作为洗脱剂,洗脱液减压浓缩后进行硅胶柱层析分离,氯仿∶甲醇(8∶2)梯度洗脱得到梓醇单体,纯度达90%以上,梓醇得率为6%。A separation technology of catalpol from Rehmannia with investigated. The content of catalpol in the extract was determined by high （HPLC）. Nine different kinds of macroporous adsorbent resins were studied and desorption, and D101 resin showed that D101 resin had the macroporpus performance adsorbent resins was liquid chromatography on the static capacity of adsorption was best for the separation of the extraction solution of Rehmannia. The results highest static adsorption capacity of 69.2mg/g can be well described by Langmuir and Freudlich equation. The 5% ethanol e dry luti resin and its isotherm curve on on removal of the solvent under reduced pressure provided a brown powder, which was subjected to an open column silica gel eluted with a CHCI3 - MeOH gradient. The fraction eluted catalpol and the purity of the compound was more than 90% purity by technology was 6%. chromatography on with CHCI3 -MeOH （ 8： 2 ） was identified as HPLC analysis. The yield of this separation

关 键 词：地黄 梓醇 大孔吸附树脂 柱层析 高效液相色谱 

分 类 号：Q819[生物学—生物工程]