去卵巢大鼠不同时期皮质骨与松质骨变化的显微CT观察  

Micro-CT analysis of cortical and cancellous bone on left tibial in ovariectomized rats

作　　者：范慧洁[1] 戴如春[1] 盛志峰[1] 方玲娜[1] 伍贤平[1] 廖二元[1] 

机构地区：[1]中南大学湘雅二医院代谢内分泌研究所,长沙市410011

出　　处：《中华老年医学杂志》2008年第1期57-61,共5页Chinese Journal of Geriatrics

基　　金：国家自然科学基金（30400514）;卫生部临床学科重点建设资助项目（2004-468-50）

摘　　要：目的应用显微CT观察去卵巢大鼠胫骨近端皮质骨与松质骨骨密度和骨微结构差异。方法40只7月龄SD大鼠,随机分为去卵巢（OVX）组和假手术（SHAM）组,每组20只,于手术后第3周及第15周处死。处死后剥离左侧胫骨,行显微CT三维扫描,扫描完成后选取距生长板远端2.5 mm、层厚0.4 mm骨组织为皮质骨感兴趣区域,选取距生长板远端0.7 mm、层厚1.2 mm骨组织为松质骨感兴趣区域行三维重建。获取二维最大密度投射图像及三维结构图像,并对感兴趣区的皮质骨和松质骨进行定量分析。结果3周时,OVX和SHAM组大鼠皮质骨面积分别为（0.43±0.13）、（0.31±0.06）mm^2;骨髓腔面积（10.31±1.98）、（8.44±1.25）mm^2,截面总面积（10.74±2.05）、（8.75±1.26）mm^2,截面惯性矩（4.10±0.73）、（3.49±0.37）mm^4,两组比较差异有统计学意义（均为P〈0.05）。第15周时,除OVX组皮质骨平均厚度低于SHAM组外（P〈0.05）,其余各参数两组比较差异无统计学意义。15周OVX组大鼠左侧胫骨骨丢失敏感区域内皮质骨平均厚度和皮质骨面积较3周OVX组大鼠下降（P〈0.05）。SHAM组15周大鼠骨内径周长、骨外径周长和截面惯性矩增大,与3周比较,差异有统计学意义（P〈0.05）。3周时,OVX和SHAM组体积骨密度分别为（288.2±48.2）mg/mm^3和（408.4±51.6）mg/mm^3、组织骨密度（604.5±45.3） mg/mm^3和（686.7±40.0）mg/mm^3、骨体积分数（25.1±5.1）%和（33.6±4.1）%、骨小梁数量（6.04±2.94）个/mm和（9.85±2.83）个/mm,两组比较差异有统计学意义（均为P〈0.05）,结构模型指数（分别为3.11±0.36）和2.58±0.36）,小梁间隔为（0.37±0.22）mm和（0.14±0.10）mm,明显高于SHAM组（P〈0.05）。第15周时,OVX组体积骨密度、骨体积分数、骨小梁数量、结构模型指数和骨小梁间隔改变与SHAM组比较,差异有统计学意义（均为P〈0.05）,但组织骨密度差别消失。OVX组大鼠15周�Objective To observe the changes of bone mass and microarchitecture in ovariectomized rat left tibia by Micro CT（μCT）.Methods Forty seven-month-old SD rats were randomly divided into ovariectomized（OVX）and sham-operated（SHAM）groups,twenty in each group.After killed at 3^rdweek and 15^thweek aftersurgery,μCT scanning were performed in the left tibia in vitro.After scanning,the regions involving 0.4 mm slice thickness and 2.5 mm distance at the far end from tibial growth plate were selected as the region of interest（ROI）for cortical bone analysis.The regions selected as ROI of cancellous analysis,were involved in 1.2 mm slice thickness and 0.7 mm distance at the far end from tibial growth plate.After three dimension（3D） reconstruction,2D images of the maximum intensity projection and pictures of 3D microarchitecture were obtained,and bone mineral density（BMD）and microarchitectural parameters were quantitively identified.Results There were significant increases in Ct.Ar〔（0.43±0.13）mm^2 vs.（0.31±0.06）mm^2〕,Ma.Ar〔（10.31±1.98）mm^2 vs.（8.44±1.25）mm^2〕,Tt.Ar〔（10.74±2.05）mm^2 vs. （8.75±1.26）mm^2〕,and Mm〔（4.10±0.73）mm^2 vs.（3.49±0.37）mm^4〕in OVX group,compared with SHAM group at the 3^rdweek（all P〈0.05）.While at the 15^thweek,only Ct.Th showed a decrease in OVX group（P〈0.05）.In OVX group,Ct.Th and Ct.Ar were lower at 15 weeks than at 3 weeks.In SHAM group,In.Pro,Ot.Pm and Mm at 15 weeks were higher than those at 3 weeks （P〈0.05）.At the 3^（rd）week OVX rats had much lower vBMD〔（288.2±48.2）mg/mm^3 vs（408.4±51.6）mg/mm3〕,tBMD〔（604.5±45.3）mg/mm^3 vs.（686.7±40.0）mg/mm^3〕,BVF〔（25.1±5.1）%vs.（33.6±4.1）%〕,and Tb.N〔（6.04±2.94）mm^（-1）vs.（9.85±2.83）mm^（-1）〕,but higher SMI〔（3.11±0.36）vs.（2.58±0.36）〕and Tb.Sp〔（0.37±0.22）vs.（0.14±0.10）〕mm than SHAM had（P〈0.05）.At the 15th week,vBMD,BVF and Tb.N were still lower in OVX than in SHAM.SMI and Tb

关 键 词：体层摄影术 X线计算机 骨密度 骨质疏松 绝经后 

分 类 号：R686[医药卫生—骨科学]