复方银杏叶片中槲皮素的含量测定  

Determination of Quercetin in Compound Ginkgo Leaves Tablets

作　　者：危华玲[1] 陈英[1] 陈晓宇[1] 李懿[1] 

机构地区：[1]广西壮族自治区人民医院,南宁530021

出　　处：《中国药师》2008年第1期12-13,共2页China Pharmacist

基　　金：广西科学基金(编号桂科基0342003-1)

摘　　要：目的:建立复方银杏叶片中槲皮素高效液相色谱测定方法。方法:采用Nova-PakC_(18)柱(4.0mm×250mm,4μm),以甲醇:0.4%磷酸(45:55)为流动相,流速为0.9ml·min^(-1),柱温:35℃,检测波长为360nm。结果:槲皮素在进样量0.0813～0.813μg范围内与峰面积线性关系良好,r=0.9998,平均回收率为98.12%,RSD为1.61%(n=6)。结论:本方法简便,准确,重复性好,可用于控制复方银杏叶片的质量。Objective： To establish the method for determining the content of quercetin in compound ginkgo leaves tablets. Method： Nova-Pak C ls column（4.0 mm ×250 mm,4 um）was used. Methanol-0.4% phosphorous acid （45：55） was used as the mobile phase. The flow rate was 0, 9 ml·min^-1, the column temperature was 35℃, and the detective wavelength was at 360 nm. Result： The linear range of quereetin was 0.081 - 0.813μg （r = 0.999 8 ）. The average recovery was 98.12% and the RSD was 1.61% （ n = 6）. Conclusion： The method is simple and accurate, and can be used reliably for the quality control of compound ginkgo leaves tablets.

关 键 词：复方银杏叶片 槲皮素 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]