大孔吸附树脂分离富集分离葶苈子中总强心苷  

Enrichment of total cardiac glycoside from Semen Descuraniae by macroporous adsorption resin

作　　者：祁琴[1] 石晋丽[1] 闫兴丽[1] 万新[1] 王爱芹[2] 王秀坤[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]北京市宣武区第三职业学校,北京100053

出　　处：《中国中药杂志》2008年第2期136-139,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30572463)

摘　　要：目的:考察6种大孔树脂对葶苈子总强心苷的分离富集分离性能,确定最佳工艺条件。方法:以总强心苷吸附量,洗脱率,洗脱物总强心苷含量为指标,采用紫外-可见分光光度法进行含量测定。结果:NKA-9型树脂对葶苈子中总强心苷具有较好的分离富集分离能力,其最佳的工艺条件为:葶苈子提取物质量浓度4 g.mL-1(生药量),最大吸附量3.54 mg.g-1,装柱径高比1/3,吸附流速2 mL.m in-1,水洗脱量3倍柱体积,洗脱剂80%乙醇,洗脱量3倍柱体积,洗脱速度1 mL.m in-1。结论:NKA-9型树脂分离富集总强心苷的最佳工艺稳定高效,可推广应用于生产。Objective： To establish the separation and enrichment technology for total cardiac glycoside from Semen descuraniae, 6 types of macroporous adsorption resin were investigated. Method： The adsorption capacity, elution ratio and purify of total cardiac glycoside were used as evaluating criteria. The UV-VIS spectrophotometric method was used in the determination of total cardiac glycoside content. Result： NKA-9 had better property of separation and purification and the optimal technological conditions were： the concentration of the sample of Semen Descuraniae, 4 g · mL^-1（ amount to crude drug） ,The maximum adsorption capacity of total cardiac glycoside, 3.54 mg · g^-1 ,the optimal column diameter/height, 1/3, the flow velocity of adsorption, 2 mL · min^-1, washing water, 3 times of resin volume, the eluting reagent, 80% ethanol （3 times of the resin volume）, eluting velocity, 1 mL · min^-1. Conclusion ： The technology of separating and purifying total cardiac glycoside of Semen Descuraniae with NKA-9 was stable and effective, and can be applied to manufacture.

关 键 词：葶苈子 总强心苷 大孔吸附树脂 

分 类 号：R284.1[医药卫生—中药学]