天山雪莲HPLC指纹图谱研究  

HPLC Fingerprint of Saussurea involucrata

作　　者：欧元[1] 袁晓凡[1] 徐春明[1] 赵兵[1] 王晓东[1] 王玉春[1] 

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室

出　　处：《中草药》2008年第1期105-108,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(20506027);中国科学院西部行动高新技术项目(KGCX2-SW-506)

摘　　要：目的建立天山雪莲药材的HPLC指纹图谱，为雪莲药材的快速质量控制提供依据。方法以绿原酸、紫丁香苷、芦丁、木犀草素、芹菜素5种对照品为对照，采用HPLC法，测定了10批次不同产地天山雪莲的色谱图，确立参照指纹图谱。色谱条件：色谱柱Diamonsil^TM C18（250mm×4．6mm，5μm），甲醇-水（0．8％醋酸，0．2％三乙胺，pH3．5-4．0）线性梯度洗脱，柱温30℃，检测波长270nm，记录时间30min，体积流量1．0mL／min。结果标示出天山雪莲的13个特征指纹峰。结论该方法准确、重现性好，为天山雪莲的指纹图谱质量控制提供了参考。Objective To establish HPLC fingerprints of Saussurea involucrata for the quick quality control. Methods Chlorogenic acid, syringin, rutin, luteolin, and apigenin were used as reference substances. Diamonsil^TM C18 （250 min×4.6 mm, 5 μm） column was used and the methanol-water （0.8% acetic acid, 0. 2% triethylamine, pH 3.5-4.0） was chosen as a mobile phase. The column temperature was 30 ℃ and the fingerprint was detected at 270 nm. The detection time was 30 min, and the flow rate was 1.0 mL/min. Ten batches of S. involucrata samples from various habitats were anlayzed to establish a reference fingerprint. Results Thirteen characteristic peaks were indicated on HPLC fingerprints. Conclusion This method is simple and accurate with a good reproducibility and provides a reference standard for the quality control of S. involucrata.

关 键 词：天山雪莲 指纹图谱 HPLC 相似度 

分 类 号：R282.7[医药卫生—中药学]