干燥方法和提取温度对板蓝根、大青叶有效成分的影响  

Effect of drying method and extracting temperature on contents of bioactive constituents in Radix Isatidis and Folium Isatidis

作　　者：董娟娥[1] 龚明贵[2] 梁宗锁[1] 王淑英 

机构地区：[1]西北农林科技大学,陕西杨凌712100 [2]中国科学院水利部水土保持研究所,陕西杨凌712100 [3]宁夏平罗县黄渠桥镇林业站,宁夏平罗753406

出　　处：《中草药》2008年第1期111-114,共4页Chinese Traditional and Herbal Drugs

基　　金：陕西省“十五”科技攻关项目(2001KG01-G15-03)

摘　　要：目的研究干燥方法和提取温度对板蓝根、大青叶有效成分量的影响，为确定板蓝根、大青叶规范化生产的干燥技术参数提供理论依据。方法采用晒干、阴干和不同温度的烘干方法干燥板蓝根、大青叶，采用不同温度水浴提取有效成分，HPLC法测定靛蓝、靛玉红的量。结果60℃烘干板蓝根、大青叶有效成分损失最少，以60℃烘干为标准，高温（90℃以上）干燥使板蓝根有效成分损失40％～60％、大青叶有效成分损失30％60％，阴干也降低了板蓝根、大青叶中的有效成分的量；采用索氏提取法、以氯仿为提取溶剂、水浴温度为80～85℃时对靛蓝、靛玉红的提取率最高，其次是90、75℃，95℃的提取率最低。结论50～80℃烘干和晒干是板蓝根、大青叶适宜的干燥方法，阴干和高温烘干的方法不可取。采用索氏提取方法、以氯仿为提取溶剂时，80～85℃水浴温度较为适宜。Objective the bioactive constituents Isatidis were dried in the To study the effect of different drying methods and extracting temperatures on in Radix Isatidis and Folium Isatidis. Methods Radix Isatidis and Folium shade, in the sun, and baked out with different temperatures; the bioactive constituents in Radix Isatidis and Foliurn Isatidis were extracted by Soxhlet extracting method under different temperatures; the contents of indigo and indirubin were determined by HPLC. Results Bioactive constituents of Radix Isatidis and Foliurn Isatidis lost less under the drying temperature of 60 ℃. The contents of the bioactive constituents reduced 40%-60% in Radix Isatidis and 30%-60% in Folium Isatidis at high drying temperature （over 90 ℃） and also reduced by dried in the shade compared with that at drying temperature of 60 ℃. The contents of the bioactive constituents were also reduced by dried in the shade ; the contents of indigo and indirubin were the highest under the extracting temperature of 80-85 ℃ by using Soxhlet extracting method with chloroform solvent, the lowest, 95 ℃. Conclusion The suitable methods for drying Radix Isatidis and Folium Isatidis are to be dried in the sun and baked under 50- 80 ℃. The temperature of water bath has significant impact on the extraction rate of the bioactive constituents while using Soxhlet extracting methods, 80-85 ℃ are suitable.

关 键 词：菘蓝 板蓝根 大青叶 干燥 靛蓝 靛玉红 

分 类 号：R282.6[医药卫生—中药学]