药用石斛遗传多样性的SRAP标记研究  

Studies on genetic diversity of medicinal Dendrobium by SRAP

作　　者：樊洪泓[1] 李廷春[2] 邱婧[1] 李正鹏[1] 林毅[1] 蔡永萍[1] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]安徽省烟草研究所,安徽凤阳233100

出　　处：《中国中药杂志》2008年第1期6-10,共5页China Journal of Chinese Materia Medica

基　　金：安徽省教育厅自然科学重点科研项目(2006KJ054A)

摘　　要：目的：利用SRAP分子标记技术进行药用石斛的遗传多样性研究。方法：采用在本实验中优化的SRAP反应体系,应用NTSYS软件对9种供试石斛的SRAP-PCR结果进行分析。结果：从中筛选得到40对多态性引物组合,共产生1 782条多态性条带,平均每个引物组合产生44.55条多态性条带,显示了较高的多态性比率。聚类分析40对引物组合的扩增结果,供试材料分为2大类,Jaccard’s遗传相似系数在0.3302~0.7892。结论：SRAP技术可有效地应用于药用石斛的遗传多样性及亲缘关系的研究。Objective： To study the genetic diversity of medicinal Dendrobium by SRAP.Method： The genetic diversity of 9 spices Dendrobium was studied by using the optimized SRAP reaction system.The NTSYS software was used to analyze the markers.Result： Forty primer pairs were selected from 88 amplified 1 782 polymorphic bands with an average of 44.55 polymorphic bands per primer pair.Cluster analysis using UPGMA method based on the data of SRAP amplified bands by 40 primer pairs showed that 9 spices of could be distinguished into two main groups.Jaccard＇s similarity coefficient ranged from 0.330 2-0.789 2.Conclusion： The results of this research indicate that SRAP molecular marker is efficient to study the medical Dendrobium genetic diversity.

关 键 词：药用石斛 遗传多样性 SRAP 

分 类 号：S567.239[农业科学—中草药栽培]