复方黄连胶囊对糖尿病肾病大鼠肾组织TGF-β_1与Ⅳ型胶原蛋白表达的影响  

Effect of compound Rhizoma Coptidis capsule on expression of transforming growth factor-β_1 and type Ⅳ collagen proteins in renal tissue of diabetic rats with nephropathy

作　　者：刘圣[1] 唐丽琴[1] 陈礼明[1] 陈象青[1] 张善堂[1] 魏瑜[1] 

机构地区：[1]安徽医科大学附属省立医院,安徽合肥230001

出　　处：《中国中药杂志》2008年第1期68-72,共5页China Journal of Chinese Materia Medica

基　　金：安徽省2001年度重点科研项目(01023018)

摘　　要：目的:探讨复方黄连胶囊(CRCC)对糖尿病肾病(DN)大鼠肾病变的保护作用及其机制。方法:以链脲佐菌素(STZ)复制早期DN大鼠模型,动物分为正常组、模型组、CRCC高、中、低剂量组(生药含量分别为4.36,2.18,1.09 g.kg-1)、消渴丸(XKW)0.80 g.kg-1治疗组,灌胃给药,每天1次,30 d后检测空腹血糖(FBG)、尿素氮(BUN)、肌酐(Cr)、胰岛素(Ins)及尿蛋白(Upro);光镜观察肾组织形态学改变;免疫组织化学检测肾小球系膜细胞转化生长因子β1(TGF-β1)与Ⅳ型胶原(Ⅳ-C)蛋白的表达。结果:CRCC可降低大鼠FBG,BUN,Cr及Upro水平,升高Ins,降低肾小球系膜细胞TGF-β1与Ⅳ-C蛋白的表达,改善肾组织形态学异常。结论:CRCC对早期DN大鼠肾微循环病变具有保护作用,延缓DN慢性病理进展,这种作用可能与其抑制肾组织TGF-β1与Ⅳ-C蛋白表达有关。Objective： To explore the effect and mechanism of compound Rhizoma Coptidis capsule（CRCC） on diabetic nephropathy in experimental rats.Method： The rat model of early diabetic nephropathy was induced by injection of streptozotocin（STZ）.The rats were divided into 6 groups： normal control group,model group,3 CRCC treatment groups and XKW treatment group.The fasting blood glucose（FBG）,blood urea nitrogen（BUN）, creatinine（Cr）,insulin（Ins） and urinary protein（Upro） were tested 30 days later.The expression of transforming growth factor-β1（TGF-β1） and type Ⅳ collagen（Ⅳ-C） proteins and the pathological changes in renal tissue of diabetic rats with nephropathy were observed by optical micrography.Result： CRCC could reduce the levels of FBG,BUN,Cr,Upro and the expression of TGF-β1 and Ⅳ-C proteins,and alleviate pathological lesion in renal tissue of diabetic rats with nephropathy.Conclusion： CRCC may protect the renal function and slow down the progression of diabetic nephropathy in rats by suppressing the expression of TGFβ1 and Ⅳ-C proteins in renal tissue.

关 键 词：复方黄连胶囊 糖尿病肾病 病理形态学 转化生长因子Β1 Ⅳ型胶原 

分 类 号：R285[医药卫生—中药学]