慢性肾功能衰竭患者血小板膜糖蛋白Ⅰb/Ⅸ/Ⅴ复合物表达的研究  

Expression of platelet membrane glycoprotein Ⅰb/Ⅸ/Ⅴ complex in patients with chronic renal failure

作　　者：秦铀[1] 沈霖[1] 卢芙蓉[1] 石威[1] 刘建国[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院中西医结合科,430022

出　　处：《实用医学杂志》2008年第1期37-39,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金资助(编号:30572441)

摘　　要：目的:观察慢性肾功能衰竭(CRF)患者血小板膜糖蛋白(GP)Ⅰb/Ⅸ/Ⅴ复合物及其组分GPⅠbα的表达与正常人之间的差异。方法:采用流式细胞术检测CRF患者血小板GPⅠb/Ⅸ/Ⅴ复合物和GPⅠbα的表达,采用比浊法检测其血小板最大聚集率(MA)。结果:CRF患者GPⅠb/Ⅸ/Ⅴ复合物、GPⅠbα表达和MA明显低于正常对照组(P=0.013,P=0.001,P=0.006);相关性分析显示GPⅠb/Ⅸ/Ⅴ复合物和GPⅠbα的表达与血肌酐呈明显负相关(P=0.041,P=0.026)。结论:血小板GPⅠb/Ⅸ/Ⅴ复合物作为凝血酶和血管性血友病因子的受体,在CRF患者体内的表达下降,导致血小板聚集功能障碍,可能是CRF出血倾向的重要原因之一。Objective To investigate the expression of platelet membrane glycoprotein （GP） Ⅰb/Ⅸ/Ⅴ complex and its component GP Ⅰbα in patients with chronic renal failure （CRF）. Methods Fifty-one patients with CRF were observed in our hospital from March 2006 to May 2007. Thirty-two healthy volunteers were taken as normal control group. The expressions of GP Ⅰb/Ⅸ/Ⅴ complex and GP Ⅰb/Ⅸ/Ⅴ were assessed by flow cytometry, and platelet maximum agglutination （MA） was determined by turbidity method. Results As compared with healthy subjects, the expressions of GP Ⅰb/Ⅸ/Ⅴ complex and GP Ⅰ bα and MA were remarkably lower（P 〈 0.05 or 0.01 ）. The expression level of GP Ⅰb/Ⅸ/Ⅴ complex and GP Ⅰ bα were negatively correlated with serum creatinine （P 〈 0.05）. Conclusion As a receptor of thrombin and yon willebrand factor, GP Ⅰb/Ⅸ/Ⅴ complex decreased in CRF patients, resulting in platelet aggregation dysfunction, which may partly explain the hemorrhagic trend in CRF patients.

关 键 词：肾功能衰竭 慢性 血小板膜糖蛋白类 血小板聚集 流式细胞术 

分 类 号：R692.5[医药卫生—泌尿科学]