生附片化学成分的HPLC／ESI-MS^n研究  

Studies on the Components of Aconitum carmichaefi by Using HPLC/ESI-MSn

作　　者：越皓[1] 皮子凤[1] 宋凤瑞[1] 刘志强[1] 刘淑莹[1] 

机构地区：[1]中国科学院长春应用化学研究所长春质谱中心

出　　处：《化学学报》2008年第2期211-215,共5页Acta Chimica Sinica

基　　金：国家自然科学基金(Nos．30472134、30672600);973项目(No．2006CB5047060)资助项目

摘　　要：采用高效液相色谱与电喷雾质谱联用技术,对生附片的化学成分进行了系统的研究.并辅以提取离子色谱方法.发现微量的化学成分.通过保留时间,质荷比及多级串联质谱数据,共鉴定了48个成分,其中双酯型生物碱8个,单酯型生物碱7个,脂型生物碱29个.其中双酯型生物碱是生附片中的主要成分,而单酯型和脂型生物碱的含量和种类较少.The components of the herbs of Aconitum carmichaeli were studied by HPLC coupled with ESI-MS^n. Extracted ion chromatograms was applied to detect low level compounds. Forty eight compounds were identified comparing on retention time, m/z and MS^n data. There were eight diester-diterpenoid alkaloids, seven monoester-diterpenoid alkaloids and twenty nine lipo-alkaloids. The relative abundance of diester-diterpenoid alkaloids were more than those of monoester-diterpenoid alkaloids and lipo-alkaloids in the herbs of Aconitum carmichaeli.

关 键 词：附子 生物碱 液质联用 

分 类 号：R284[医药卫生—中药学]