甘薯近缘野生种Ipomoea trifida(4x) GISH分析  

GISH Analysis of Sweet Potato Wild Relative Ipomoea trifida (4x)

作　　者：向素琼[1] 汪卫星[1] 李晓林[1] 陈瑶[1] 郭启高[1] 何桥[1] 梁国鲁[1] 

机构地区：[1]西南大学园艺园林学院,重庆北碚400716

出　　处：《作物学报》2008年第2期341-343,共3页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30600417)

摘　　要：以甘薯近缘野生种I.trifida(2x)为探针,与I.trifida(4x)2个株系"695104"和"697288"的体细胞染色体进行基因组荧光原位杂交,结果显示,2株系都与I.trifida(2x)有很近的亲缘关系,但2株系的信号存在差异。"695104"几乎所有染色体整条都有均匀明亮的信号,应为I.trifida(2x)基因组直接加倍而来;而"697288"与"695104"不同,虽然各条染色体也均有杂交信号,但信号的区域与亮度有差异,较为复杂,可分为三种情况。第1种是整条染色体有均匀明亮的信号,亮度与分布区域同"695104",有41条;第2种是几乎整条染色体有信号,但亮度较第一种暗,有14条;第3种为染色体部分区域有信号,亮度较前二者更暗,有5条。推测"697288"是在加倍同时或之后又发生了基因组重组与部分变异。Using genomic DNA ofL trifida （2x） as probe, L trifida （4x） lines 695104 and 697288 were analyzed with genome in situ hybridization （GISH）. The results showed that the two liness were very closely related to I. trifida （2x） based on hybridization signals. Bright and even hybridization signals were observed on all chromosomes of 695104, showing that I. trifida （4x） 695104 should be from doubling of I. trifida （2x） chromosome set. Hybridization signals of 697288 differed from those of 695104 in brightness and region size. It displayed there types： the first was that 41 chromosomes appeared bright and even signals on whole chromosomal; the second was that 14 chromosomes presented even signals almost on whole chromosome but weaker than the first; the third was that 5 chromosomes displayed more weaker signals than those of both the first and the second. Therefore 697288 might derived from L trifida （2x）, but through recombination and variation.

关 键 词：甘薯 近缘野生种 基因组原位杂交(GISH) 

分 类 号：S531[农业科学—作物学] S512.1