系统性红斑狼疮骨髓受累机制初步研究  

Study on the pathogenesis of blood cytopenia in patients with systemic lupus erythematosus

作　　者：付蓉[1] 上原灯纪子[2] 巩路[2] 邵宗鸿[1] 

机构地区：[1]天津医科大学总医院血液肿瘤科,300052 [2]天津医科大学总医院感染免疫科,300052

出　　处：《中华血液学杂志》2008年第1期48-51,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金（30670886）;天津市高等学校科研基金（200317）

摘　　要：目的初步探讨系统性红斑狼疮（SLE）骨髓受累的可能机制。方法对21例初治SLE患者骨髓标本21份，10例正常对照骨髓标本分别进行骨髓单个核细胞（BMMNC）Coombs试验并采用甲基纤维素法检测CFU—E、CFU—GM、BFU—E产率。结果10份正常对照骨髓标本BMMNC—Coombs试验均为阴性，21例SLE患者，有12例（57．1％）阳性，其中17例合并血细胞减少者中有10例（58．2％）阳性；4例血细胞正常者中有2例（50．0％）阳性，SLE患者BMMNC—Coombs试验阳性率显著高于正常对照组（P〈0．05）。而SLE合并血细胞减少组与血细胞正常组相比，阳性率差异无统计学意义（P〉0．05）。BMMNC抗体类型与血细胞水平无显著相关性。SLE合并血细胞减少组的CFU—E、CFU—GM产率与正常对照组相比，差异无统计学意义（P〉0．05）。SLE血细胞正常组CFU—E、CFU—GM产率显著高于正常对照组（P〈0.05）。SLE患者中，合并血细胞减少组CFU—E、CFU—GM产率显著低于血细胞正常组（P〈0．05）。SLE患者无论是否合并血细胞减少其BFU—E产率均显著低于正常对照组（P值均〈0．05）。而SLE合并血细胞减少组与血细胞正常组患者相比，其BFU—E产率差异无统计学意义（P〉0．05）。结论SLE患者BMMNC膜表面被覆自身抗体；造血祖细胞增殖、分化功能无明显异常，某些SLE患者可能通过造血祖细胞的代偿增殖维持正常的血细胞水平。Objective To explore the pathogenetic mechanism of cytopenia in the patients with systemic lupus erythematosus （SLE）. Methods Twenty-one patients with SLE and 10 healthy controls were enrolled in this study. Bone marrow mononuclear cells （BMMNC） Coombs test was used to determine the autoantibodies and in vitro CFU-E, BFU-E and CFU-GM yields were used to determine the proliferation function of hematopoietic stem/progenitor cell （ HSC ）. Results Positive results of BMMNC-Coombs test were observed in 12 patients with SLE （57.1%）, among them, 10 with （58.2%） and 2 without blood cytopenia. The yields of CFU-GM（49 ±40）, and CFU-E（49 ±35） in SLE patients without cytopenia were higher than that in normal controls, but lower than that in cytopenia SLE patients. The BFU-E yield in SLE patients （3 ± 4） was significantly lower than that in normal controls（36 ± 12）. Conclusion There were auto-antibodies on BMMNC in patients with SLE. The blood cytopenia in SLE patients maybe resulted from the destructions of bone marrow hematopoietic cells by the autoantibodies. The capacities of bone marrow HSC in SLE patients for proliferation compensation of hematopoietic cell destruction were not damaged.

关 键 词：红斑狼疮 系统性 自身抗体 骨髓 集落形成单位测定 

分 类 号：R686[医药卫生—骨科学]