一氧化氮对骨关节炎滑膜细胞转化生长因子-β表达的影响  

The effect of nitric oxide on the expression of transforming growth factor-beta of osteoarthritis synoviocytes

作　　者：江建明[1] 刘传芳[1] 梁鹏展[1] 孙炜[2] 

机构地区：[1]南方医科大学附属南方医院脊柱骨病科,广州510515 [2]深圳市第二人民医院骨科

出　　处：《中华风湿病学杂志》2008年第2期79-82,146,共5页Chinese Journal of Rheumatology

基　　金：国家自然科学基金资助项目（30571890）

摘　　要：目的了解一氧化氮（NO）对骨关节炎（OA）滑膜细胞转化生长因子（TGF）-β表达的影响，并分析它们之间的相关性。方法OA患者滑膜细胞培养，收集不同分组[空白组、脂多糖（LPS）组、氨基胍（AG）组、LPS＋AG组]的滑膜细胞培养上清液，检测其NO、TGF—β含量并行统计分析。结果LPS诱导下，滑膜细胞增加NO的表达，而TGF—β表达下调，AG可以抑制NO的表达，提高TGF—β的表达，NO与TGF—β存在负相关。结论NO抑制OA滑膜细胞TGF—β的表达，TGF—β可能是OA的一种保护因子。Objective To investigate the effect of nitric oxide （NO） on the expression of transforming growth factor-β （TGF-β） on osteoarthritis （OA） synoviocytes and analyse their correlation. Methods The supematants of cultures of different groups [control, lipopolysaccharide （LPS）,aminoguanidine （AG）, LPS＋ AG） of OA synoviocytes were collected. The concentration of NO and TGF-β was detected and statistical analysis was performed. Results The expression of NO was increased in the group of synoviocytes induced by LPS but the expression of TGF-β was decreased. AG could inhibit the expression of NO and increased the expression of TGF-β. There was a negative correlation between NO and TGF-β. Conclusion NO can inhibit the expression of TGF-β on OA synoviocytes. TGF-β maybe a protect factor of OA.

关 键 词：一氧化氮 转化生长因子 骨关节炎 滑膜细胞 

分 类 号：R285.5[医药卫生—中药学]