Study on the specific interaction between angiogenin and aptamer by atomic force microscopy (AFM)  

作　　者：HE XiaoXiao JIN Rong YANG Liu WANG KeMin LI Wei TAN WeiHong LI HuiMin 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics, Institute of Life Science and Biological Technology, Engineering Center for Biomedicine, College of Material Science and Technology, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Changsha 410082, China

出　　处：《Chinese Science Bulletin》2008年第2期198-203,共6页

基　　金：the National Key Basic Research Program (Grant No. 2002CB513100-10);Key Technologies Research and Development Program (Grant No. 2003BA310A16);Key Project Foundation of China Education Ministry (Grant No. 107084);Program for New Century Excellent Talents in University (Grant No. NCET-06-0697);National Natural Science Foundation of China (Nos. 90606003 and 20405005);Outstanding Youth Foundation of Hunan Province (Grant No. 06JJ10004)

摘　　要：The specific interaction between angiogenin and aptamer has been investigated by using AFM. The specificity of the interaction is revealed by comparing the binding probability of aptamer to other ele- ments in a series of control experiments. The results have shown that there is specific interaction force between angiogenin and aptamer. Moreover, the single molecular pull-off force between angiogenin and aptamer has also been determined using the Poisson statistical method to be 133.7±11.7 pN. These findings obtained are helpful to the better revelation of recognition mechanism between angiogenin and aptamer, which provided basis for further understanding the inhibition of the aptamer to angio- genic activity.The specific interaction between angiogenin and aptamer has been investigated by using AFM. The specificity of the interaction is revealed by comparing the binding probability of aptamer to other elements in a series of control experiments. The results have shown that there is specific interaction force between angiogenin and aptamer. Moreover, the single molecular pull-off force between angiogenin and aptamer has also been determined using the Poisson statistical method to be 133.7±11.7 pN. These findings obtained are helpful to the better revelation of recognition mechanism between angiogenin and aptamer, which provided basis for further understanding the inhibition of the aptamer to angiogenic activity.

关 键 词：原子力显微术 血管生成素 交互作用 力检测 

分 类 号：R4[医药卫生—临床医学]