碳、氧化锌和碳载氧化锌复合纳米颗粒对小鼠胚胎成纤维细胞活性抑制及DNA损伤的研究  

Study of C-ZnO composite nanoparticles on viability inhibition and DNA damage of mouse embryo fibroblasts

作　　者：杨辉[1] 刘超[1] 杨丹凤[1] 张华山[1] 袭著革[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《卫生研究》2008年第1期12-15,共4页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No30500399)

摘　　要：目的探讨不同化学组成纳米颗粒对小鼠胚胎成纤维细胞(MEF)的毒性效应及机制。方法选择纳米碳、纳米氧化锌、碳载氧化锌复合纳米颗粒制备细胞染毒悬液,三种纳米颗粒分别设立5个剂量组(5、10、20、50和100μg/ml)对BALB/c小鼠胚胎成纤维细胞进行24h、48h、72h染毒培养;利用细胞形态学观察和噻唑蓝实验(MTT比色法)检测上述三种纳米颗粒对细胞活性的影响;通过单细胞凝胶电泳(彗星试验)检测低剂量纳米颗粒对细胞DNA的损伤作用。结果三种纳米颗粒能够明显影响MEF细胞的生长形态,对细胞活性的抑制作用具有明显的时间-效应和剂量-效应关系;纳米碳、纳米氧化锌及复合纳米颗粒的半数致死浓度分别为21.85、21.94和23.02μg/ml;随染毒剂量升高,纳米氧化锌颗粒对细胞活性的抑制作用显著增强;低剂量水平上,复合纳米颗粒造成的DNA损伤最为明显。结论化学组成不同的纳米颗粒能够对MEF细胞造成毒性损伤;当暴露剂量达到一定水平时,纳米颗粒的金属成分含量可能是其毒性作用的主导因素。Objective To study the cytotoxicity and DNA damage induced by C-ZnO composite nanoparticles in Mouse embryo fibroblasts（MEF） cells. Methods MEF cells were treated with the suspension of carbon nanoparticles, zinc oxide nanoparticles and composite nanoparticles at the concentrations of 5,10,20,50,100μg/ml. Cellular morphology and MTT assay were carried out to evaluate the viability of cells treated with particles for 24h, 48h and 72h respectively. Single cell gel electrophoresis （SCGE, comet assay）was performed to assess DNA damage induced by nanoparticles at the doses of 5μg· ml-^1 and 10μg·ml^-1 . Results Three types of nanoparticles could induce morphologic change and viability inhibition with significant logarithmic time-effect and dose-effect relationship. Median lethal concentrations （ IC50 ） of carbon nanoparticles, zinc oxide nanoparticles and composite nanoparticles were 21.85,21.94 and 461.10μg/ml respectively. The inhibitory effect on cellular viability of Zinc oxide nanoparticles increased significantly with the dose enhances. The most DNA damage was induced by composite nanoparticles at lower concentrations. Conclusion Nanoparticles with different chemical composition induced distinct cytotoxicity and DNA damage in MEF cells. The metal content of composite nanoparticles played an important role in the toxic effect.

关 键 词：纳米氧化锌 纳米碳 小鼠胚胎成纤维细胞 细胞毒性 DNA损伤 环境污染 

分 类 号：X513[环境科学与工程—环境工程] R994.6[医药卫生—毒理学]