福建省烟粉虱自然种群Wolbachia感染特点  

Characteristics of Wolbachia infection in natural populations of Bemisia tabaci in Fujian province

作　　者：林煌真[1] 李正西[1] 

机构地区：[1]中国农业大学昆虫学系,北京100094

出　　处：《昆虫学报》2008年第1期14-19,共6页Acta Entomologica Sinica

基　　金：国家重点基础研究发展计划项目(2006CB102006);国家自然科学基金项目(30571218)

摘　　要：Wolbachia是专性的细胞内细菌,广泛存在于节肢动物生殖组织。已有的研究结果表明,节肢动物中存在A组和B组Wolbachia,而烟粉虱Bemisia tabaci中主要检测到了B组Wolbachia。本研究从福建省采集到17个不同烟粉虱地理种群,首先通过rDNA-ITS1克隆测序鉴定了不同烟粉虱地理种群的生物型,然后采用Wolbachia 16S rDNA的特异引物,并通过PCR-RFLP技术分析了不同烟粉虱地理种群中Wolbachia的感染特点。结果表明:从福建省闽侯、平潭、南平、来舟、漳平和沙县采集到的烟粉虱自然种群属于非B型,而非B型烟粉虱种群中存在广泛的超感染现象,即单个非B型烟粉虱个体中同时感染了不同型Wolbachia。相反,B型烟粉虱自然种群的个体中只感染A组Wolbachia。该研究依据密集采样的数据进一步证实了Wolbachia在烟粉虱自然种群中的分布确实与宿主的生物型密切相关,提示Wolbachia可能在烟粉虱的种群分化中发挥作用。Wolbachia are obligate intracellular symbionts that infect reproductive tissues of a wide range of arthropod species. Previous reports showed that arthropods carried Wolbachia belonging to supergroup A and B, but Bemisia tabaci mainly harbored Wolbachia of supergroup B. In this study, 17 natural populations of B. tabaci collected from Fujian province were firstly molecularly identified based on rDNA-ITS1 sequences, and then specific primers of Wolbachia 16S rDNA were used to amplify a target region through polymerase chain reaction, which was used for restriction fragment length polymorphism analysis. The results demonstrated that whitefly populations from areas including Minghou, Pintan, Nanping, Laizhou, Zhangping and Shaxian of Fujian province belonged to non-B biotypes, while the other populations belonged to B biotype. Wolbachia superinfection was commonly detected in non-B biotypes of B. tabaci based on PCR-RFLP analysis, while in B biotype of B. tabaci only supergroup A Wolbachia infection was found. These data, based on dense sampling, further verified that Wolbachia infection was closely related to the biotypes of B. tabaci, suggesting that Wolbachia might play a role in population differentiation of B. tabaci.

关 键 词：烟粉虱 种群分化 WOLBACHIA 超感染 RDNA PCR-RFLP 

分 类 号：Q965[生物学—昆虫学]