基于线粒体ND2基因的中国斑翅蝗科部分种类分子系统学研究(直翅目:蝗总科)  

Molecular evolution and phylogenetic analysis of some species of Oedipodidae (Orthoptera:Caelifera) in China based on complete mitochondrial ND2 gene

作　　者：丁方美[1] 黄原[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062

出　　处：《昆虫学报》2008年第1期55-60,共6页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(30470238)

摘　　要：本文的目的是通过对斑翅蝗科部分种类的线粒体ND2基因进行分析,重建斑翅蝗科昆虫的系统发育关系,并探讨分子系统发育关系和传统分类结果的异同。扩增并测定了我国斑翅蝗科10属16种蝗虫的线粒体ND2全基因1023bp的序列,对序列的碱基组成、转换颠换、系统发育信号等进行了分析。并基于ND2全基因序列数据,分别采用邻接法(NJ)、最简约法(MP)、最大似然法(ML)和贝叶斯法重建了10属16种蝗虫的系统发育关系。结果表明:斑翅蝗科蝗虫ND2全基因A+T含量平均为74.6%;痂蝗亚科和异痂蝗亚科没能得到区分,建议合并为一个亚科;而斑翅蝗亚科和飞蝗亚科的分类地位还存在争议。The aim of the present study was to reconstruct the phylogenetic relationships of Oedipodidae and to explore the differences of the results between the phylogenetic relationships and the tradition taxonomy. The ND2 gene complete sequences （1 023 bp） of 10 genera 16 species of Oedipodidae were amplified and sequenced. Nucleotides composition, transition and transversion of this gene were analyzed. By using Neighbor Joining （N J）, Maximum Parsimony （MP）, Most Likehood （ML） and MrBayes （BI） methods, we reconstructed the molecular phylogeny of Oedipodidae based on ND2 gene sequence. The results indicated that average A ＋ T content of ND2 gene in Oedipodidae was 74.6%, showing a strong A ＋ T bias; Bryodema and Bryodemella were not separated in this study, so we considered it was appropriate to merge them as one subfamily; the status of Oedipodidae and Locustinae was still problematic.

关 键 词：斑翅蝗科 ND2基因 系统发育 分子进化 

分 类 号：Q965[生物学—昆虫学]