生防枯草芽孢杆菌L1特性的初步研究  

Preliminary characterization of Bacillus subtilis strain L1

作　　者：李永刚[1] 宋兴舜[2] 赵雪莹[1] 马凤鸣[1] 

机构地区：[1]东北农业大学农学院,哈尔滨150030 [2]东北林业大学生命科学学院遗传系,哈尔滨150040

出　　处：《植物保护》2008年第1期57-61,共5页Plant Protection

基　　金：黑龙江省博士后科研基金(LRB06-299);农业部寒地作物生理生态重点开放实验室项目

摘　　要：通过对峙培养，测定出枯草芽孢杆菌L1的抑菌谱较宽，特别是对水稻纹枯病菌、大豆菌核病菌、禾谷镰孢菌、辣椒灰霉病菌、玉米小斑病菌抑菌效果明显；枯草芽孢杆菌L1不同发酵时间经湿热灭菌处理后，5d发酵液中抑菌活性物质含量最高，对水稻纹枯病菌菌丝生长抑制率达83．23％，发酵液随时间延长抑菌效果不再增加；枯草芽孢杆菌L1抑菌活性物质对温度不敏感；枯草芽孢杆菌L1发酵液用硫酸铵梯度沉淀法提取粗蛋白在硫酸铵饱和度达60％～70％（不含60％）沉淀的活性物质抑菌效果最好，对水稻纹枯病菌平均抑菌半径达1．15；枯草芽孢杆菌L1对玉米、大豆、小麦、番茄、菜豆、黄瓜、水稻无致病性，而且还有保鲜和促生作用。Bacillus subtilis strain L1 （BSL1） was examined for its inhibitory range to various pathogenic fungi by using dual culture. The results showed that its inhibitory efficacy to Rhizoctonia solani, Sclerotinia sclerotiorurn, Fusariurn grarninearurn, Botrytis cinerea and Bipolaris maydis was obvious. The contents of the antimicrobial substances secreted by BSL1 were determined at different fermentation time after moist heat sterilization. The contents of the antimicrobial substances after 5 days were the highest, accounting for 83. 23%, but their activity didn＇t change with the increase of time. Meanwhile, the activity of the antimicrobial substances was insensitive to temperature. The crude proteins were extracted from the fermented liquids using gradient precipitation method with ammonium sulfate. The inhibitory efficacy of the antimicrobial substances under saturation of ammonium sulfate was 60%- 70%, and the average radius was 1. 15cm. BSL1 had no pathogenicity to corn, soybean, wheat, tomato, kidney bean, cucumber and rice.

关 键 词：枯草芽孢杆菌 抑菌物质 粗蛋白 致病性 

分 类 号：S482.292[农业科学—农药学]