椎体终板下微循环障碍直接导致椎间盘退变的实验研究  

Subendplate microcirculation disturbance directly contributes to intervertebral disc degeneration

作　　者：郑召民[1] 吕游[1] 陈辉[1] 刘辉[1] 张奎渤[1] 李佛保[1] 

机构地区：[1]中山大学附属第一医院脊柱外科,广州510080

出　　处：《中华外科杂志》2008年第3期213-216,共4页Chinese Journal of Surgery

摘　　要：目的通过建立椎体终板下微循环障碍动物模型，探讨椎间盘退变发病的可能机制。方法将24只新西兰白兔随机分为实验组和对照组，实验组采用联合应用内毒素与激素的方法制备典型椎体终板下微循环障碍模型，并通过终板微血栓染色证实；对照组为阴性空白对照，不给予任何药物干扰，仅标准饲料喂养。3个月后分析实验组和对照组动物椎间盘的水含量、生化成分含量和组织形态学，从而评估椎间盘的退变程度。结果终板微血栓染色证实实验组成功构建椎体终板下微循环障碍模型，3个月后实验组动物椎间盘水含量、生物化学成分含量均低于对照组，椎间盘切片染色可见椎间盘退变的表现。结论椎体终板下微循环障碍可直接导致椎间盘退变，椎间盘营养供给障碍是椎间盘退变的发病机制之一。Objective To build subendplate microcirculation disturbance animal model and to investigate the potential pathogenesis of intervertebral disc degeneration （IVDD）, Methods Twenty four New Zealand white rabbits were divided into treatment group （Group A） and control group （Group B）, In Group A, animals received endotoxin and corticosteroid application to build subendplate microcirculation disturbance animal model, validated by microthrombus staining, In Group B, animals were given no drug, but standard feeding. After 3 month, the extent of IVDD was evaluated by the water content, biochemistry analysis, and morphology, Results Subendplate microthrombus staining confirmed the exist of microcirculation disturbance. The water content and biochemistry components content of disc in Group A were lower than those of disc in Group B, and IVDD was observed in morphology. Conclusion Subendplate microcirculation disturbance can directly contribute to IVDD, the nutrients diffusion barrier is the potential pathogenesis of IVDD,

关 键 词：椎间盘 微循环 椎体终板 椎间盘退变 

分 类 号：R686[医药卫生—骨科学]