大鼠血管新生内膜形成过程中NF-κB的活化和ICAM-1及COX-2表达的变化  

Activation of NF-κB and expression of ICAM-1 and COX-2 genes in rat neointimal formation

作　　者：韩梅[1] 温进坤[1] 刘月平[1] 宋伟[1] 

机构地区：[1]河北医科大学基础医学研究所,河北省医学生物技术重点实验室,河北石家庄050017

出　　处：《中国病理生理杂志》2008年第2期257-260,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30472167);河北省自然科学基金资助项目(No.C2005000722)

摘　　要：目的:探讨大鼠血管内膜增生过程中NF-κB活化与内膜炎性增生的相互关系。方法:Westernblotting分析血管壁中NF-κB p65、IκBα及其下游炎性基因ICAM-1和COX-2的表达;用免疫沉淀分析检测NF-κB p65的苏氨酸磷酸化。结果:内皮剥脱后,血管总蛋白与核蛋白中p65的含量于术后7 d达峰值;术后21 d下降但仍高于0、1 d组;但胞浆p65含量无明显变化。p65苏氨酸磷酸化水平与NF-κB核转位成负相关关系。IκBα于术后1 d表现出一过性降低,术后14 d开始回升,21 d接近正常组水平;与此相反,ICAM-1和COX-2的表达于内皮剥脱后升高,至14 d时达峰值,21 d时表达量下降但仍高于正常组。结论:血管内皮剥脱诱导的内膜增生过程中伴有持续的NF-κB p65活化和炎性因子的表达。AIM ： To investigate the relationship between NF-κB activation and intimal hyperplasia. METHODS ： The protein extract from aorta was used to detect the expression of NF-κB p65, IκBα, ICAM - 1 and COX - 2 by Western blotting, and detection of threonine phosphorylation of p65 was performed by immunoprecipitation analysis. RESULTS ： The level of p65 reached a maximum level at day 7, and then decreased significantly, which was higher than that in the control, in both cellular extract and nuclear extract at day 21 after balloon injury. The p65 level in cytoplasm had no obvious changes at different time points after balloon injury. The threonine phosphorylation of NF-κB p65 was negative correlated with the nuclear translocation of NF-κB. IκBα level showed a 15% decrease at day 1 after balloon injury, compared with control group, then returned at day 14, and reached to normal level at day 21. However, the expression of ICAM - 1 and COX -2 was maximal at day 14, and then declined but higher than that in control group at day 21 after balloon injury. CONCLUSION ： The activation of NF-κB p65 and the expression of ICAM - 1 and COX - 2 are parallel to the neointimal thickening after balloon injury.

关 键 词：内皮 血管 NF-ΚB 血管内膜 大鼠 胞间黏附分子1 环氧合酶2 

分 类 号：R543.1[医药卫生—心血管疾病] Q556[医药卫生—内科学]