体外构建气管黏膜上皮组织的研究  

Reconstruction of tracheal epithelium mucosa tissue in vitro with a tissue engineering method

作　　者：吴明明[1] 崔鹏程[1] 赵大庆[2] 郑德育[1] 段小红[1] 

机构地区：[1]第四军医大学唐都医院耳鼻咽喉科,陕西西安710038 [2]第四军医大学基础部病理学教研室,陕西西安710033

出　　处：《第四军医大学学报》2008年第3期208-210,共3页Journal of the Fourth Military Medical University

基　　金：陕西省自然科学基金(2006C224)

摘　　要：目的:应用组织工程原理和方法在体外构建气管黏膜上皮组织.方法:将原代兔气管上皮细胞种植在铺有鼠尾胶原的小肠黏膜下层(SIS)上培养,在体外构建气管黏膜上皮组织,培养1wk后行HE染色,对气管上皮细胞行免疫细胞学鉴定.结果:原代气管上皮细胞培养1wk后增殖旺盛,10d时上皮细胞分布范围更广.将构建的气管黏膜上皮组织行HE染色,显示有气管黏膜上皮和SIS双层结构.抗角蛋白单克隆抗体免疫细胞化学反应阳性显色率可达90%左右.结论:将铺有鼠尾胶原的SIS复合气管上皮细胞生长,成功的在体外构建出组织工程化的气管黏膜上皮组织.AIM： To reconstruct tracheal epithelium mucosa tissue in vitro with tissue engineering method. METHODS： The rabbit tracheal epithelium cells were cultured primarily and characterized by photocontrast microscopy. Then the cultured epithelium cells were seeded onto collagen-coated small intestinal submueosa （SIS）. After 1 week, the cells were identified with HE staining. RESULTS： Many epithelium cells with good proliferation property were found in the culture system after 1 week. Epithelial cells distributed widely on day 10. The two layers of the epithelium and SIS were observed by HE staining. Antikeratin was positive in over 90% of the cells. CONCLUSION： Compound tracheal epithelium mueosa tissue is reconstructed in vitro successfully.

关 键 词：支气管/细胞学 上皮细胞 小肠黏膜下层 组织培养 组织工程 

分 类 号：R318.13[医药卫生—生物医学工程]