AQP4与Kir4.1在大鼠眼内组织的共表达  

Co-localization of AQP4 and Kir4.1 in the rat eyes

作　　者：陈海[1] 孙善全[1] 汪克建[1] 陈通[1] 谭戈[1] 杨美[1] 

机构地区：[1]重庆医科大学神经生物学研究室,重庆400016

出　　处：《第四军医大学学报》2008年第3期214-216,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30470608);高校博士学科点专项科研基金(20040631002)

摘　　要：目的:探讨AQP4与Kir4.1在大鼠眼内的定位分布及二者的共表达.方法:用免疫组织化学及激光扫描共聚焦显微技术检测AQP4与Kir4.1在大鼠眼内的分布.结果:免疫荧光显示,AQP4与Kir4.1在大鼠视网膜和睫状体内的分布形式相似;免疫荧光双标显示,AQP4与Kir4.1在视网膜Mller细胞及睫状突无色素上皮细胞的顶膜面及基底膜面呈重叠分布.结论:AQP4与Kir4.1在大鼠视网膜Mller细胞及睫状突无色素上皮细胞共表达,提示二者可能具有协同作用,参与房水的产生以及视网膜内水、K+运输平衡的调节.AIM： To investigate the distribution and co-localization of AQP4 and Kit4. 1 in the frozen sections of rat eyes. METHODS： The indirect immunofluoresence was used to, detect the distribution of AQP4 and Kit4.1 in the rat eyes, and double immunofluorescence was used to determine their co-localization. RESULTS： AQP4 and Kit4. 1 were distributed together in the retinal neural layers and the ciliary non-pigment epithelial cells of rat eyes. The double labeling of AQP4 and Kit4. 1 showed that AQP4 and Kit4.1 were co-localizated in the membrane of Müller cells and the basal membrane or the topic membrane of ciliary non-pigment epithelial cells. CONCLUSION： This co-localization of AQP4 and Kit4.1 in the rat eyes suggests their coupling functions in the generation of aqueous fluid and the process of water and K^＋ transporting balance of Müller cells.

关 键 词：睫状体/细胞学 视网膜 水通道蛋白 钾通道 内向整流 免疫荧光组织化学与荧光双标记 

分 类 号：R339.14[医药卫生—人体生理学]