葡萄糖对人肝细胞血管生成素样蛋白3表达的影响  

Effect of glucose on angiopoietin-like protein 3 expression in human liver cells

作　　者：李倩[1] 王继红[1] 程梅[1] 王欣[1] 曾建涛[1] 

机构地区：[1]重庆医科大学生物化学与分子生物学教研室,重庆400016

出　　处：《第四军医大学学报》2008年第3期248-251,共4页Journal of the Fourth Military Medical University

基　　金：重庆医科大学创新基金(CX200528)

摘　　要：目的:观察高浓度葡萄糖对人肝细胞株(L02)及胰岛素抵抗细胞模型(IR-L02)的血管生成素样蛋白3(ANGPTL3)表达的影响,探讨ANGPTL3与糖尿病(DM)并发高脂血症的关系,在细胞水平分析DM并发高脂血症的分子机制.方法:以L02为研究对象,并建立胰岛素抵抗模型(IR-L02).L02和IR-L02两组细胞分别在含5.6,7.0,11.1,28.0和33.0mmol/L葡萄糖的培养液中培养,5.6mmol/L组作为对照组.用RT-PCR方法检测ANGPTL3的mRNA表达量,并用WesternBlot方法检测ANGPTL3蛋白质表达水平,分析ANGPTL3与DM并发高脂血症的关系.结果:高浓度葡萄糖可促进L02和IR-L02两组细胞ANGPTL3的mRNA和蛋白质表达,并呈一定的量效关系,变化差异均有统计学意义(P<0.0001);IR-L02细胞对葡萄糖的刺激尤为敏感(P<0.0001).结论:高浓度葡萄糖可上调ANGPTL3的基因和蛋白质表达,其在DM并发高脂血症中可能起重要作用.AIM： To investigate the effect of glucose on angiopoientin-like protein 3 （ANGPTL3） expression in human liver cells （L02） and insulin resistant L02 cells （ IR-L02）, and to explore the relation of ANGPTL3 with the development of diabetes mellitus accompanied with hyperlipoproteinemia and the molecular mechanism of diabetes mellitus complicated with hyperlipoproteinemia at the cell level. METHODS： The model of IR-L02 was established. The L02 cells and IR-L02 cells were incubated separately in mediums containing 5.6, 7.0, 11.1, 28.0 and 33.3 mmol/L glucose, among which 5.6 mmol/L glucose group was taken as control. The relative level of ANGPTL3 mRNA expression was detected by reverse transcription-polymerase chain reaction （RT-PCR）. The relative level of ANGPTL3 protein was measured by Western Blot. The relation of ANGPTL3 with diabetes mellitus complicated with hyperlipoproteinemia was also analysed. RESULTS： High concentration of glucose stimulated ANGPTL3 mRNA and protein expresion both in L02 and IR-ID2 in a linear dose-effect relationship. The differences were considered significant （ P 〈 0. 0001 ）. Compared with L02, IR-L02 was more sensitive to the glucose stimulation （ P 〈 0. 0001 ）. CONCLUSION： High concentration of glucose upregulates ANGPTL3 expression at the levels of both gene and protein. ANGPTL3 may play an important role in diabetes mellitus complicated with hyperlipoproteinemia.

关 键 词：血管生成素样蛋白3 糖尿病 高脂血症 葡萄糖 胰岛素抗药性 

分 类 号：R58[医药卫生—内分泌] Q51[医药卫生—内科学]