血清中萘呋胺的反相高效液相色谱-荧光测定  

RP HPLC FLUORIMETRY OF NAFTIDROFURYL IN SERUM

作　　者：余琛[1,2] 张慧[1,2] 洪有采[1,2] 崔桂仙 夏粉英 

机构地区：[1]上海市徐汇区中心医院 [2]上海天平制药厂

出　　处：《中国医药工业杂志》1997年第7期311-313,共3页Chinese Journal of Pharmaceuticals

摘　　要：以盐酸维拉帕米为内标，建立了血清样品中萘呋胺的反相高效液相色谱－荧光测定法。血清样品经二次液－液反相萃取后，得到了较好的纯化。萘呋胺和内标的提取回收率高于７６％，日内、日间精密度分别为２．２０％和３．５８％。Naftidrofuryl in serum was determined with RP HPLC and fluorimetry, using verapamil hydrochloride as internal standard (IS), after pretreatment Nova Pak C18 (4 μm) column (150 ×3.9 mm ID) was used. Mobile phase was methanol water triethylamine formic acid (60∶40∶0.02∶0.02). The detection was performed at 277 nm (Ex) and 335 nm (Em). The detection limit was 0.2 ng (S/N≥3). The extraction recovery of naftidrofuryl and IS during pretreatment as over 76%. The coefficients of variation were 2.20 % (within day) and 3.58% (between days).

关 键 词：萘呋胺 血清 高效液相色谱 含量测定 

分 类 号：R972.4[医药卫生—药品]