重组人肝再生增强因子对梗阻性黄疸大鼠肝细胞线粒体DNA的保护作用  

Beneficial Effects of Recombinant Human Augmentation Factor of Liver Regeneration on Hepatocyte mtDNA in Obstructive Jaundice Rats

作　　者：唐春[1] 谢斌[1] 别平[1] 李昆[1] 张玉君[1] 马正伟[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所肝胆外科,重庆400042

出　　处：《现代生物医学进展》2008年第2期201-204,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助(30571806)

摘　　要：目的:初步探讨重组人肝再生增强因子(rhALR)保护及改善梗阻性黄疸大鼠肝细胞线粒体功能及肝功能的机制。方法:144只健康wistar大鼠随机分为SHAM组,BDO-RBF组,BDO-RBF-rhALR组,利用荧光定量PCR方法,对各组大鼠肝细胞总mtDNA、缺失型mtDNA进行相对定量检测结果:胆道梗阻后,肝细胞线粒体总mtDNA拷贝数出现明显下降(P<0.01),BDO-RBF-rhALR组总mtDNA拷贝数下降程度明显低于BDO-RBF组(P<0.05);对于缺失型mtDNA占总mtDNA的百分比,在SHAM组,未检测出缺失型mtDNA,而在BDO-RBF组及BDO-RBF-rhALR组,均可见缺失型mtDNA,BDO-RBF组的缺失型mtDNA占总mtD- NA的百分比明显高于BDO-RBF-rhALR组(P<0.05);在胆道梗阻解除后,BDO-RBF-rhALR组的总mtDNA的拷贝数及缺失型mtDNA修复速度明显快于BDO-RBF组(P<0.05)结论:rhALR可通过保护及修复梗阻性黄疸大鼠肝细胞受损的mtDNA,达到保护及改善梗阻性黄疸大鼠肝细胞线粒体功能及肝功能的目的。Objectives： To study the molecular mechanism of the beneficial effects of recombinant human augmentation factor of liver regeneration on mitochondrial function and liver function in obstructive jaundice rats. Methods： One hundred and forty four Wistar rats were randomly divided into three groups, namely SHAM, BDO-RBF, and BDO-RBF-rhALR. Quantify the changes of the whole mtDNA, deletion form mtDNA at selected time points in each group. Results： Compared to SHAM group, the copies of whole mtDNA reduced in obstructive jaundice rats （P〈0.01）. Compared to BDO-RBF group, the copies of whole mtDNA reduced lighter in BDO-RBF-rhALR group （P〈0.05）. The deletion form mtDNA was found in BDO-RBF and BDO-RBF groups, but not in SHAM group. There were more the percentage of deletion form mtDNA in whole mtDNA in BDO-RBF group than BDO-RBF-rhALR group （P〈0.05）. The copies of whole mtDNA and the percentage of deletion form mtDNA in whole mtDNA in BDO-RBF-rhALR group recovered faster than BDO-RBF group after restoration of bile flow （P〈0.05）. Conclusion： The recombinant human augmentation factor of liver regeneration protects mitochondrial and liver function because it can protect and repair damaged mtDNA in obstructive jaundice rats.

关 键 词：重组人肝再生增强因子 梗阻性黄疸 线粒体DNA 

分 类 号：R657.43[医药卫生—外科学] Q291[医药卫生—临床医学]