AFM对黄精赞育胶囊优选方处理前后大鼠弱精子超微结构的观察研究  

Observation of Ultrastructure of Weak Sperms of Rats before/after Treatment of Huangjingzanyu Optimized Formula by Atomic Force Microscopy

作　　者：陈斌 杨景国[1] 孟繁林[1] 韩冬[2] 刘保兴[3] 王琦[3] 

机构地区：[1]北京市普仁医院泌尿男科,北京100062 [2]国家纳米科学中心纳米医学与生物学研究室,北京100080 [3]北京中医药大学生殖医学研究中心,北京100029

出　　处：《现代生物医学进展》2008年第2期229-232,245,共5页Progress in Modern Biomedicine

基　　金：北京市优秀人才培养资助项目

摘　　要：目的:应用原子力显微镜技术实现对大鼠精子超微结构的实时成像,对比观察中药作用前后弱精子超微结构的变化。方法:制作少弱精症大鼠模型;运用非接触模式原子力显微镜技术对比观察正常精子和病理性精子在中药作用前后超微结构的动态变化。结果:获得精子头体、颈部和鞭毛等部位的实时超微结构图像。结论:AFM可直接探测精子头体的各种畸形,实现对精子全貌的观测。通过修复弱精子超微结构的病理形态学缺陷可能是黄精赞育胶囊优选方改善弱精子质量的机制之一。Objective： To investigate ultrastructural changes, particularly at the surface, that occur in pathological spermatozoa by using atomic force microscopy （AFM） and to examine the morphological alterations responsible for infertile sperms treated with Huangjignzanyu optimized formula. Methods： The pathological sperms with asthenozoospermia were obtained from the rat model. Normal fertile and pathological spermatozoa treated with Huangjignzanyu optimized formula were examined by using a conventional AFM in a non-contact mode. Results： Morphological details, topological information, and three-dimensional images of the head, neck, and flagellum were presented for both normal and pathological sperms. Even the ultrastructure at the top of the flagellum and the region of the acrosome cap were clearly distinguishable. Conclusions： This study has significant importance not only for identifying spermatozoa alterations but also for understanding morphological defects and their effects on infertility. The whole ultrastructural form of infertility sperms could be improved by Huangjignzanyu optimized formula. By this experiment, AFM should be applied to study the rat sperms as an important novel tool.

关 键 词：原子力显微镜 黄精赞育胶囊优选方 弱精子 超微结构 

分 类 号：R698[医药卫生—泌尿科学]