超声提取桦褐孔菌子实体多糖及多糖对癌细胞作用的研究  

Effect on Cancer Cell Proliferation of Polysaccharide Extracted from Inonotus obliquus Fruit Bodies Using Ultrasound

作　　者：张慧丽[1] 杨松[2] 寇巍[1] 辛茹[1] 李玉[1] 刘宏生[1] 

机构地区：[1]辽宁大学生命科学院,沈阳110036 [2]沈阳军区药品检验所,沈阳110026

出　　处：《食用菌学报》2007年第4期51-54,共4页Acta Edulis Fungi

摘　　要：采用正交试验优化桦褐孔菌子实体多糖的超声提取工艺条件,并对提取多糖的抗肿瘤活性进行研究。结果表明,桦褐孔菌多糖超声提取的最佳工艺条件为超声时间70min,温度80℃,频率20kHz,加水量12倍水,其多糖在体外对肝癌HEPG2细胞株具有明显的抑制作用,并可诱导肿瘤细胞凋亡,体内实验发现多糖可显著抑制小鼠S180肉瘤的生长,并具有增强免疫作用。Optimal conditions for extraction of polysaccharide from Inonotus obliquus fruit bodies （IOP） were determined as follows： extraction time, 70 min; temperature, 80℃ ; ratio of aqueous solvent to fruit body, 12 ： 1; and ultrasound frequency, 20 kHz, lOP at a concentration of 1.44 μg/mL inhibited cancer cell growth by-65% as measured using a tetrazolium-based （MTT） assay to evaluate the anti-proliferative effect of the polysaccharide on hepatocarcinoma HEPG2 cells. A 27% and 35% reduction in tumor weight was also observed in S-180 tumor-bearing mice administered low （50 mL/kg.d, 8 μg/mL） and high （100 mL/kg·d, 16 μg/mL） doses of lOP, respectively.

关 键 词：桦褐孔菌子实体多糖 正交实验 超声提取 肝癌细胞 吞噬指数K 

分 类 号：R285.5[医药卫生—中药学] R284[医药卫生—中医学]