水线草提取物的体外抗结肠癌作用及其机理初探  

Antitumor Effects and Mechanism of the Extracts from Oldenlandia corymbosa

作　　者：旷丽莎[1] 江炜[2] 丛蓉[1] 史薇[1] 梅兵[1] 侯爱君[2] 钱旻[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062 [2]复旦大学药学院,上海200032

出　　处：《天然产物研究与开发》2007年第B05期22-25,共4页Natural Product Research and Development

基　　金：上海-SK研究与发展基金项目（2004008-t）

摘　　要：分别用水、石油醚、正丁醇和乙酸乙酯萃取水线草乙醇提取物得五个组分。MTT检测法、Wright染色法及TUNEL染色法初步研究各萃取部位对人结肠癌细胞株HCT-8、RKO的体外抑制作用。其中石油醚萃取部位及沉淀部位在浓度低至31．3μg／mL时对两种人结肠癌细胞株均有一定程度的杀伤作用。水线草中具有抗结肠癌活性的化学物质的分子结构及作用机理值得进一步深入研究。To observe the anti-tumor effects and mechanism of different extracts from Oldenlandia corymbosa at the cell level, MTr method was used to detect the anti-tumor effects of the extracts,while Wright dye and TUNEL assays were used to investigate the anti-tumor mechanism. ResuLts show that the petroleum ether fraction and the precipitation from the ethanol extract of Oldenlandht corymbosa showed inhibitory effects on HCT-8, RKO cell lines in vitro with the concentrations of 31.3 μg/mL. They can lead to the conclusion that there are antitumor constituents in the extracts of O. corymbosa,which can provide the basis for the research and development of new anti-tumor drugs.

关 键 词：水线草 粗提物 结肠癌 抗肿瘤作用 细胞凋亡 

分 类 号：R285[医药卫生—中药学] Q946.91[医药卫生—中医学]