The Expression Levels of Endogenous aFGF mRNA in Microwave Burn Wound Tissues and Its Clinical Significance  

作　　者：李亦武 李贵玲 

机构地区：[1]School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology [2]The Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2007年第5期576-578,共3页华中科技大学学报（医学英德文版）

基　　金：This project was supported by a grant from Foundation of National 863 Program for Research and Development of Class I Novel Drug rh-aFGF (No 2002AA2Z3349)

摘　　要：The expression levels and changes of endogenous acid fibroblast growth factor （aFGF） in microwave burn wound tissues were detected in order to investigate how to get better therapeutic effects by using the exogenous aFGF for repairing trauma. A burnt-wound animal model was established by NS-FⅡ multifunction spectrum therapeutics equipment, and reverse transcriptase-polymerase chain reaction （RT-PCR） and immunohistochemistry assay were applied to detect the expression levels of endogenous aFGF mRNA in microwave burn wound tissues. The expression level of endogenous aFGF mRNA was significantly increased in the burn wound tissues 12 h after burn, reached the peak at 48 h, and gradually deceased 96 h after burn. The expression of endogenous aFGF mRNA after tissue damage was reversible, and its intensity was in accordance with the repair process of tissue damage, suggesting endogenous aFGF may take part in the cell metabolism and proliferation, and then promote the repair of the burn wound.The expression levels and changes of endogenous acid fibroblast growth factor （aFGF） in microwave burn wound tissues were detected in order to investigate how to get better therapeutic effects by using the exogenous aFGF for repairing trauma. A burnt-wound animal model was established by NS-FⅡ multifunction spectrum therapeutics equipment, and reverse transcriptase-polymerase chain reaction （RT-PCR） and immunohistochemistry assay were applied to detect the expression levels of endogenous aFGF mRNA in microwave burn wound tissues. The expression level of endogenous aFGF mRNA was significantly increased in the burn wound tissues 12 h after burn, reached the peak at 48 h, and gradually deceased 96 h after burn. The expression of endogenous aFGF mRNA after tissue damage was reversible, and its intensity was in accordance with the repair process of tissue damage, suggesting endogenous aFGF may take part in the cell metabolism and proliferation, and then promote the repair of the burn wound.

关 键 词：aFGF：  repair in trauma expression of endogenous aFGF mRNA 

分 类 号：R644[医药卫生—外科学]