TaqMan MGB双探针方法检测慢性乙型肝炎病毒YMDD变异的研究  

TaqMan MGB Dual-probe Detecting YMDD Motif Mutation of Hepatitis B Virus in Chronic Infected Patients

作　　者：陈占国[1] 陶志华[1] 周武[1] 戴美洁[1] 刘媚娜[1] 余忠新[1] 

机构地区：[1]温州医学院附属第一医院实验诊断中心,浙江温州325000

出　　处：《中华医院感染学杂志》2008年第2期171-173,共3页Chinese Journal of Nosocomiology

摘　　要：目的探讨TaqMan MGB双探针方法检测慢性乙型肝炎病毒YMDD变异的可靠性。方法采用荧光定量PCR法检测HBV DNA含量,TaqMan MGB双探针方法和测序方法检测其YMDD变异,以测序方法为参考方法,评价TaqMan MGB双探针方法检测慢性乙型肝炎病毒YMDD变异的可靠性。结果246例HBV DNA阳性患者血清标本中,TaqMan MGB双探针方法阳性检出率为100%,100例正常对照全部为阴性;YMDD无突变106例,YMDD有突变(YIDD变异+YVDD变异)140例,与核酸测序方法比较,符合率为100%;该方法经过敏感度实验,最低检出下限为1×103拷贝/ml。结论TaqMan MGB双探针方法能便捷、灵敏、特异地检测YMDD基序变异情况,适用于临床检测慢性乙型肝炎患者拉米夫定治疗的YMDD基序变异。OBJECTIVE To evaluate the accuracy of TaqMan MGB dual-probe detecting YMDD motif mutation of hepatitis B virus in chronic infected patients. METHODS The concentrations of serum HBV DNA were measured by fluorometric real-time PCR. YMDD motif mutations of HBV were detected by TaqMan MGB dual-probe and sequence analysis respectively. Sequence analysis test was regarded as a reference method. RESULTS Among the 246 cases serum samples, all were positive for HBV-DNA by TaqMan MGB dual-probe, while those in 100 healthy people were negative. There were 106 cases with wildtype of YMDD motif mutations, 140 cases with mutants with YIDD and YVDD. It was competely accordant with the results by sequence analysis. The sensitivity of TaqMan MGB dual-probe was 1 ×10^3 copies/ml. CONCLUSIONS TaqMan MGB dual-probe has the superiority of high sensitivity, high specificity, convenience and quickness. It is reliable to monitor YMDD motif mutation for chronic hepatitis B infected patients treated with lamivudine.

关 键 词：乙型肝炎病毒 变异 拉米夫定 YMDD 

分 类 号：R512.62[医药卫生—内科学]