微生物比浊法检定抗生素G-418效价  

Determination of the potency of antibiotic G-418 by microbial turbidimetry

作　　者：杨天赐[1,2] 彭宣宪[3] 

机构地区：[1]厦门大学附属中山医院 [2]厦门大学生命科学学院,厦门361005 [3]厦门大学生命科学学院

出　　处：《中国抗生素杂志》2008年第2期90-92,共3页Chinese Journal of Antibiotics

基　　金：科技部科技创新基金资助(04C26213510157)

摘　　要：采用微生物比浊法检定抗生素G-418的效价。在本实验的浓度范围内(3.76μg/ml～8.77μg/ml),标准曲线线性方程为Y=-0.9184X+0.9917,回归方程为Y=-0.9215X+0.9927,r=-1.0002,其浓度与吸收度的线性关系良好,标准曲线成立。样品检测可靠性测验,回归显著(P<0.05),S、T为平行直线,实验结果可靠。采用微生物比浊法测定G-418的效价,具有耗时短,灵敏度高,操作简便,不需要特殊设备,且人为影响因素少等诸多特点,值得推广应用。A microbial turbidimetry was used to determine the potency of antibiotic G-418. The calibration curve was inear in the range of 3.76U/ml-8.77U/ml, while the calibration curve linear equation is Y= - 0. 9184X ＋ 0. 9917 and the regression equation is Y= - 0. 9215 X ＋ 0. 9927, r = - 1. 0002. The reliability of the method was discussed as well. The result is reliable with significant regression （ P〈0. 05） and S and T parallel lines. The result indicates that the method of microbial turbidimetry can be used more widely because of its convenience, accuracy and quickness.

关 键 词：G-418 微生物比浊法 效价 

分 类 号：R978.12[医药卫生—药品]