振荡磁场下超顺磁性链霉素PELA微球体外药物释放特征探索  

Regulation of drug release from super paramagnetic chitosan streptomycin PELA microspheres in blood clot of rabbit by oscillating magnetic fields and the mechanism discussion

作　　者：罗聪[1] 安洪[1] 蒋电明[1] 杨晓兰[2] 朱照静[2] 赖国旗[2] 

机构地区：[1]重庆医科大学附属第一医院,重庆400016 [2]重庆医科大学,重庆400016

出　　处：《中国抗生素杂志》2008年第2期101-105,共5页Chinese Journal of Antibiotics

基　　金：国家高技术研究发展计划863计划(课题编号:2002AA326020)

摘　　要：目的制备超顺磁性硫酸链霉素-聚乳酸-聚乙二醇(PELA)微球(superparamagnetic chitosan streptomycin PELA micro-spheres,spCSPM),研究此微球的特性,并对其在振荡磁场作用下体外药物释放规律进行研究。方法用化学共沉淀法合成纳米超顺磁Fe304壳聚糖纳米粒(superparamagnetic chitosan Fe3O4 nanospheres,spFCN),再用双乳化(W/O/W)溶剂蒸发法制备spCSPM。将spCSPM混合入兔血中形成血凝块,在37℃模拟体液中进行体外药物溶出试验,用振荡磁场干预,用酶联免疫法(ELISA)检测硫酸链霉素的释放量。结果振荡磁场能够增加spCSPM血凝块中链霉素释放速率,与非磁性的壳聚糖聚乳酸-聚乙二醇(PELA)微球(chitosan streptomycin PELA microspheres,CSPM)相比,26d时使药物释放量提高3倍左右。结论spCSPM具有药物缓释功能,振荡磁场可重复性增加体系中药物的溶出,此体系药物缓释周期超过三周。Objective To evaluate the value of super paramagnetic chitosan nanospheres （spCSPM）, the super paramagnetic chitosan streptomycin PELA microspheres （spCSPM） were prepared and characterized. The regula tion release of spCSPM in blood clot of rabbit by oscillating magnetic fields in vitro were studied in this paper. Methods The nanospheres of super paramagnetic chitosan were prepared by chemical coprecipitation and then the super paramagnetic PELA streptomycin microspheres were prepared by solvent evaporation with the compound latex. The drug release test of spCSPM in blood clot of rabbit was done in simulation body fluid with or without oscillating magnetic fields, and streptomycin was measured with ELISA. Results Data showed that oscillating magnetic fields could promote streptomycin release of spCSPM repeatedly, Compared with chitosan streptomycin PELA microspheres （CSPM）, the amount of cumulative release was increased by three times at the 26th day. Conclusion The oscillating magnetic fields could promote drug release of spCSPM repeatedly and the periods of controlled release of this system was more than 3 weeks.

关 键 词：壳聚糖 聚-DL-乳酸-聚乙=醇共聚物(PELA) 超顺磁性微球 振荡磁场 控释 

分 类 号：R943[医药卫生—药剂学]