自体外周血干细胞移植治疗下肢动脉缺血性疾病的临床应用  

Transplantation of Autologous Peripheral Blood Stem Cells for The Treatment of Lower Limb Arterial Ischemic Disease

作　　者：缪健航[1] 师天雄[1] 胡锡祥[1] 孙健鸣[1] 张明光[1] 

机构地区：[1]广东省中山市人民医院普外二科,中山528403

出　　处：《中国普外基础与临床杂志》2008年第2期106-108,共3页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨自体外周血干细胞移植技术治疗下肢动脉缺血性疾病的可行性及存在的问题。方法自2004年3月至2007年2月期间，我院采用自体外周血干细胞技术治疗16例患有严重下肢动脉缺血性疾病的患者，通过采用重组粒细胞集落刺激因子动员骨髓干细胞增殖并释放入血，在动员的第5～6d，通过CS-3000 PLUS血细胞分离机进行外周血干细胞采集，并将采集到的浓缩干细胞液做缺血肢体肌肉注射。结果经治疗后3～24个月的追踪随访，息肢静息痛缓解，溃疡愈合，间歇性跛行时间及距离延长。结论应用自体外周血干细胞移植技术治疗下肢动脉缺血性疾病是可行且有效的，是治疗缺血性疾病的一种新手段，但该治疗方法处在临床治疗的初级阶段，尚需进一步研究和探讨。Objective To evaluate the clinical efficacy of transplantation of autologous peripheral blood stem cells （PBSC） for the treatment of lower limb arterial ischemic disease. Methods From March 2004 to February 2007, 16 patients with severe lower limb arterial ischemic disease were treated with autologous PBSC transplantation. Recombinated granulocytecolony stimulating factor （G-CSF） was used to mobilize the proliferation of bone marrow stem cells and then the stem cells were released into peripheral blood. After 5-6 days, PBSC were collected by CS-3000 PLUS blood-cells separator. Such concentrated stem cells fluid was intramuscularly injected into the ischemic areas of the lower limbs. Results The results of 3 to 24-month following-up after the transplantation of stem cells showed that the resting pains of the affected limb were greatly relieved, and ulcers were healed. The distance and duration of intermittent limping became farther and longer. Conclusion Transplantation of autologous PBSC would be a novel and effective method for the treatment of arterial ischemic disease. However, this method is still at the stage of initial clinical application, so it still need to be further studied.

关 键 词：干细胞 动脉缺血性疾病 下肢 

分 类 号：R543[医药卫生—心血管疾病]