大黄素对HK-2细胞周期增殖的影响  

Effect of emodin on HK-2 cells proliferation

作　　者：王青秀[1] 吴纯启[1] 杨红莲[1] 荆淑芳[1] 解跃华[1] 金城[2] 肖小河[2] 廖明阳[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850 [2]解放军302医院全军中药研究所

出　　处：《毒理学杂志》2007年第6期440-443,共4页Journal of Toxicology

基　　金："十五"国家科技攻关计划课题-2004BA721A14

摘　　要：目的研究大黄素对人肾小管上皮细胞系HK-2细胞的毒性和细胞周期的影响。方法体外培养HK-2细胞,利用噻唑蓝法(MTT)评价大黄素对HK-2细胞增长抑制的IC50,观察不同浓度药物对细胞形态和培养液乳酸脱氢酶(LDH)活力的影响,利用流式细胞仪检测大黄素对HK-2细胞周期的影响。结果大黄素作用HK-2细胞48 h后,明显抑制细胞的增值,其IC50值为130.65μmol/L,并且能够导致细胞发生皱缩和空泡化,LDH漏出率增加,同时对细胞周期产生阻滞作用,随着浓度的增高,G0/G1期细胞比例逐渐下降。而S期细胞明显升高。结论大黄素在体外对于HK-2的增殖具有明显的抑制作用,而这种作用可能是通过改变了HK-2细胞正常的细胞周期来实现的。Objective To investigate the cytotoxic and cell cycle blocking effects of the emodin component on human proximaltubular epithelial cell line（ HK-2）. Methods MTT assay was used to evaluate the IC50 of emodin on HK-2 cells. The changes of cell morphology and lactate dehydrogenase（ LDH）leakage were observed. The cell cycle were detected with flow eytometer analysis. Results Emodin markedly inhibited the proliferation of HK-2 cells after 48 hours culture, and its IC50 is 130.65 μmol/L. Emodin could cause cells shrinkage and vacuolation, increase LDH leakage. Emodin blocked the cell cycle with the increase of concentration, the percentage of cells in S phase were gradually increased and that in G0/G1 phase decreased. Conclusion Emodin can prevent HK-2 cell from proliferation significantly in vitro probably by changing the normal cell cycle of HK-2 cells.

关 键 词：大黄素 HK-2细胞 细胞周期 

分 类 号：R99[医药卫生—毒理学]