盐酸戊乙奎醚联合氯解磷定治疗混配农药中毒的研究  

Study on treatment of mixed agricultural chemicals poisoned by penehyclidine hydrochloride combined with pralidoxime chloride

作　　者：林大伟[1] 菅向东[1] 阮艳君[1] 郭广冉[1] 宁琼[1] 王玉彩[1] 张建平[2] 

机构地区：[1]山东大学齐鲁医院风湿职业病科 [2]山东大学医学院

出　　处：《毒理学杂志》2007年第6期460-463,共4页Journal of Toxicology

基　　金：山东省科技厅计划项目(B200357)

摘　　要：目的观察盐酸戊乙奎醚注射液(长托宁)联合氯解磷定治疗混配农药中毒的治疗效果。方法选取成年健康雄性Wistar大鼠随机分为对照组、染毒非治疗组6、h治疗组1、2 h治疗组和24 h治疗组,实验结束后留取动物血清冻存待检测。结果染毒非治疗组大鼠病死率为100%,治疗组大鼠全部存活,差异有统计学意义(P<0.01)。染毒非治疗组大鼠血清BUN、Cr、ALT、AST、ALP和GLU较对照组明显升高(P<0.01),而治疗组各时段大鼠血清BUN、Cr、ALT、AST、ALP和GLU明显低于染毒非治疗组(P<0.01)。染毒非治疗组与治疗组各时段大鼠血清TP、ALB、A/G较对照组明显降低(P<0.01)。染毒非治疗组大鼠血清CHE活力较对照组明显降低(P<0.01)。而治疗组各时段大鼠血清CHE活力明显高于染毒非治疗组(P<0.01)。治疗组各时段大鼠血清CHE活力明显低于对照组(P<0.01)。染毒非治疗组大鼠血清TNF-α水平较对照组明显升高(P<0.01)。而治疗组各时段大鼠血清TNF-α水平明显低于染毒非治疗组(P<0.01)。结论长托宁联合氯解磷定治疗混配农药中毒初步显示具有较好的疗效。Objective To observe the treatment effect of penehyclidine hydrochloride combined with pralidoxime chloride on mixed agricultural chemicals poisoning. Methods All 50 healthy adult Wistar rats were randomizedly assigned into normal control groups, paraquat group, 6 h treatment group, 12 h treatment group and 24 h treatment group. Serum blood urea nitrogen（ BUN）, creatinine（Cr）, alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, alkaline phosphatase （ALP）, total protein （ TP ）,albumin（ALB）, albumin/globulin（A/G）, glucose（GLU） and cholinesterase（CHE） was detected at the end of experiment. Serum was detected by automatic biochemistry analyzer and serum TNF-α was detected by ELISA. Results The paraquat group animal were died within six hours. None animal died in treatment group and control group. The serum BUN, Cr, ALT, AST, ALP, GLU of paraquat were higher than that of control groups（ P 〈 0.01 ）. The serum BUN, Cr, ALT, AST, ALP, GLU of treatment groups was lower than that of control groups（ P 〈 0.01）. The serum CHE of paraquat groups was lower than that of control groups （P 〈 0.01 ）. The serum CHE of treatment groups was higher than that of paraquat groups （ P 〈 0.01 ）. The serum CHE of treatment groups was lower than that of control groups（ P 〈 0.01）. The serum TNF-α of paraquat groups was higher than that of control groups（ P 〈 0.01 ）. The serum TNF-α of treatment groups was lower than that of paraquat groups （ P 〈 0.01 ）. Conclusion The treatment of penehyclidine hydrochloride combined with pralidoxime chloride is effective on mixed agricultural chemicals poisoning.

关 键 词：盐酸戊乙奎醚 氯解磷定 混配农药 中毒 治疗 

分 类 号：R99[医药卫生—毒理学]