Elementary Flux Mode Analysis for Optimized Ethanol Yield in Anaerobic Fermentation of Glucose with Saccharomyces cerevisiae  

作　　者：许晓菁 曹利民 陈询 

机构地区：[1]Department of Biochemical Engineering, School of Chemical Engineering ＆ Technology, Tianjin University, Tian jin 300072, China

出　　处：《Chinese Journal of Chemical Engineering》2008年第1期135-142,共8页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China （No.2002AA647040）

摘　　要：Elementary flux mode （EFM） analysis was used in the metabolic analysis of central carbon metabolism in Saccharomyces cerevisiae based on constructed cellular network. Calculated from the metabolic model, the ethanol-producing pathway No. 37 furthest converts the substrate into ethanol among the 78 elementary flux modes. The in silico metabolic phenotypes predicted based on this analysis fit well with the fermentation performance of the engineered strains, KAM3 and KAMll, which confirmed that EFM analysis is valid to direct the construction of Saccharomyces cerevisiae engineered strains, to increase the ethanol yield.基本流动模式(EFM ) 分析基于构造细胞的网络在 Saccharomyces cerevisiae 在中央碳新陈代谢的新陈代谢的分析被使用。从新陈代谢的模型，计算生产乙醇的小径号码 37 远在 78 个基本流动模式之中把底层变换成乙醇。在里面基于这分析预言的新陈代谢的显型与设计紧张， KAM3 和 KAM11 的发酵表演适合很好的 silico，它证实了那 EFM 分析是有效的指导 Saccharomyces cerevisiae 的构造设计紧张，增加乙醇收益。

关 键 词：elementary flux mode analysis metabolic phenotype redox balance Saccharomyces cerevisiae ETHANOL 

分 类 号：TQ24[化学工程—有机化工]