植物病毒RNA在片检测方法的建立研究  

Using RNA Glass Slide Hybridization for the Detection of Plant virus

作　　者：谷宇[1] 杜志游[2] 郎秋蕾[2] 陈集双[2] 何农跃[3] 

机构地区：[1]南京中医药大学,江苏南京210029 [2]浙江大学微生物分子生物学实验室,浙江杭州310029 [3]东南大学分子电子学国家重点实验室,江苏南京210096

出　　处：《化工时刊》2008年第1期21-24,共4页Chemical Industry Times

基　　金：江苏省社会公益研究项目(批准号:BM2003701);国家自然科学基金(批准号:60571032)

摘　　要：报道了一种检测植物病毒核糖核酸(RNA)的新方法——RNA玻片杂交法。这种方法把互补脱氧核糖核酸(cDNA)芯片技术与RNA斑点杂交技术相结合,将植物样品的总RNA直接固定在玻片上,用荧光标记的病毒特异探针与芯片杂交后分析杂交信号以确定相应的样品有无该病毒侵染。参照膜杂交的方法,我们设计了一系列实验,对芯片的制备和检测条件进行了摸索,基本建立了在玻片上杂交检测RNA病毒和类病毒的方法。A new method to detect plant RNA viruses and viroid--RNA glass slide hybridization was reported. This method combined dot blot hybridization technique and cDNA microarray technique. RNA samples extracted from new leaves of plants were spotted onto a silanated glass slide directly and then performed the hybridization on the slide with CY5 - labeled probe of virus. From the results of hybridization, sample was infected by the virus. Factors affecting the results of hybridization were demonstrated by performing large numbers of conditional experiments.

关 键 词：植物病毒检测 RNA玻片杂交 斑点杂交 

分 类 号：S432.41[农业科学—植物病理学]