脱氢表雄酮对人脐静脉内皮细胞CD40/CD40L表达的影响  

Effect of DHEA on Expression of CD40/CD40L in Human Umbilical Vein Endothelial Cells

作　　者：夏尊恩[1] 李艳[1] 汪明[1] 潘小平[1] 

机构地区：[1]武汉大学人民医院检验科,武汉430060

出　　处：《微循环学杂志》2008年第1期39-41,44,共4页Chinese Journal of Microcirculation

摘　　要：目的:探讨脱氢表雄酮(DHEA)对干扰素-γ(I NF-γ)刺激下的人脐静脉内皮细胞(HUVECs)CD40/CD40L表达的影响。方法:原代培养人脐静脉内皮细胞,给予I NF-γ刺激和不同浓度DHEA干预。采用流式细胞术检测CD40/CD40L在细胞表面的表达,通过反转录-聚合酶链反应(RT-PCR)检测CD40/CD40L mRNA的表达。结果:I NF-γ刺激HUVECs表达CD40/CD40L,DHEA下调I NF-γ诱导的HUVECs表面CD40/CD40L的表达,同时对I NF-γ刺激下的CD40/CD40L mRNA的表达有抑制作用,并且呈剂量依赖性。结论:DHEA能减轻I NF-γ刺激下的人脐静脉内皮细胞CD40/CD40L的表达。Objective:To investigate the influence of dehydroepiandrosterone(DHEA)on expression of CD40/CD40L in human umbilical vein endothelial cells(HUVECs)induced by INF-γ.Method:HUVECs were incubated with INF-γ for 24 hours with or without pretreated by DHEA.Expression of CD40/CD40L were detected by flow cytometric technology and mRNA expression were evaluated by reverse transcription polymerase chain reaction(RT-PCR).Results:It was observed that INF-γ increased the expression of CD40 and CD40L.DHEA could decrease the expression of CD40/CD40L on HUVECs induced by INF-γ.Meanwhile,DHEA inhibited CD40/CD40L mRNA expression with a dose-dependent manner.Conclusion:DHEA can inhibit the expression of CD40/CD40L in HUVECs induced by INF-γ.

关 键 词：CD40L表达 脱氢表雄酮 人脐静脉内皮细胞 雄激素水平 流行病学调查 动脉粥样硬化 脂质代谢异常 独立危险因子 

分 类 号：R725.631[医药卫生—儿科] R339.38[医药卫生—临床医学]