一株鸡新城疫病毒的分离鉴定及其油乳剂灭活苗免疫效果评价  

Isolation and Identification of a Newcastle Disease Virus and Evaluation of the Virus Oil Emulsion Vaccine

作　　者：尤永君[1] 王茹英[1] 朱小甫[1] 王永建[1] 刘志强[1] 

机构地区：[1]瑞普(保定)生物药业有限公司,河北保定071000

出　　处：《中国家禽》2008年第3期17-19,共3页China Poultry

摘　　要：从河南某种鸡场发病鸡群中分离出1株毒株,经HA、HI和动物回归试验,确定分离的病毒为NDV,命名为HNYD株。经过测定,HNYD株鸡胚半数致死量(ELD50)为109.0/0.1mL;鸡胚最小致死量的平均致死时间(MDT)为53.8h;对1日龄SPF雏鸡脑内致病指数(ICPI)为1.86,提示HNYD株为强毒力株。血清交叉反应试验显示HNYD株和LaSota株存在一定的抗原性差异。用HNYD株油乳剂灭活苗对HNYD株和LaSota株的保护率分别为90%和100%,明显优于LaSota株油乳剂灭活苗,提示HNYD株制备的疫苗对流行毒株和经典毒株的攻击均能很好的保护。A Newcastle disease virus （NDV）isolated from Henan chicken farms was determine by HA test,HI test and animals regression test,named HNYD strain. ELD50,MDT and ICPI of HNYD strain were 10^90/0.1 mL,53.8 h and 1.86, respectively,indicating HNYD strain was virulent strain. Serum cross-reaction tests showed that antigen character of HNYD strain and La Sota strain had small differences. The protection rates of HNYD oil emulsion vaccine against HNYD and La Sota strain were 90% and 100%,which was significantly better than La Sota strain oil emulsion vaccine,it suggested that HNYD strain vaccine could protect chicken against epidemic and classical NDV strains well.

关 键 词：新城疫 毒力测定 油乳剂灭活苗 

分 类 号：S858.315.3[农业科学—临床兽医学] S858.31[农业科学—兽医学]