鹅生长激素基因内含子2多态性分析  

Polymorphism on Intron 2 of Growth Hormone Gene in Goose

作　　者：赵文明[1] 陈清[1] 程金花[1] 吴信生[1] 王学斌[1] 乔娜[1] 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《中国家禽》2008年第1期24-26,共3页China Poultry

基　　金：江苏省六大人才高峰项目;江苏省农业综合开发科技推广项目[苏农开科(2006)3号]

摘　　要：利用PCR-SSCP方法对籽鹅、皖西白鹅、浙东白鹅和狮头鹅4个地方鹅种进行了单核苷酸多态性分析,共检测到4个突变位点,即360处A/G、474处C/G、551处T/C和554处C/T,产生EE、EF和FF三种基因型。χ2检验结果表明:4个鹅种均处于Hardy-Wenberg平衡状态(P>0.05),籽鹅基因型频率的分布与浙东白鹅差异显著(P<0.05),与狮头鹅差异极显著(P<0.01),皖西白鹅基因型频率的分布与狮头鹅差异显著(P<0.05)。This study investigated single nucleotide polymorphism（SNP）of growth hormone（GH）gene in 4 local goose breeds （Zi goose,Wanxi White goose,Zhedong white goose and Shitou goose）by PCR-SSCP method. 4 mutations were found among four breeds,which were 360 （A/G）,474 （C/G）,551 （T/C） and 554 （C/T）,and there were three kinds of genotypes （EE,EF and FF）. The results of Х^2 test showed that the frequencies of GH gene fit with Hardy-Weinberg equilibrium in 4 breeds. The genotype frequency of Zi goose showed significant difference with Zhedong white goose and Shitou goose,and the genotype frequency of Wanxi white goose was significant difference with Shitou goose.

关 键 词：鹅 GH基因 PCR—SSCP 

分 类 号：S828[农业科学—畜牧学] S828.2[农业科学—畜牧兽医]