家蚕幼虫关联基因在胚胎期的表达(英文)  

Larva-relevant genes expression in embryonic development stages of domesticated silkworm(Bombyx mori L.)

作　　者：陈金娥[1] 杨惠娟[1] 李建营[1] 沈彦伟[1] 钟伯雄[1] 

机构地区：[1]浙江大学动物科学学院

出　　处：《浙江大学学报（农业与生命科学版）》2008年第1期38-44,共7页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：National Basic Research Program “973” of China(2005CB121003);National Natural Science Foundation of China(30271004)

摘　　要：用蛋白质表达谱分析技术研究家蚕胚胎发育时期的基因表达.用蛋白质双向电泳技术(2-DE)分离蛋白质,用I mage Master Elite图谱分析软件比较分析12个家蚕胚胎发育特异时期的2-DE图谱,发现一些蛋白斑点丰度高,并且一旦出现就在后续特异时期一直表达,推测这些蛋白可能在胚胎发育和代谢方面起重要作用,从中选择5个点分析它们的序列和功能.用基质辅助激光解吸电离质谱(MALDI-TOF-MS)和数据库检索鉴定蛋白质,发现5个幼虫期功能蛋白分别在4个胚胎发育特异时期表达:保幼激素结合蛋白(J HBP)基因在缩短期表达,表皮生长因子受体在头胸分化期表达,家蚕幼虫表皮蛋白和副腺特异肽在毛瘤发生期表达,淀粉酶在转青期表达.这些幼虫功能蛋白都在胚胎特定的生理期功能需要下出现,表明幼虫关联基因在胚胎期有序而及时地表达.Gene expression in embryonic stages of silkworm was investigated through analysis of protein expression profile. Proteins from embryonic stages were separated by using two-dimensional electrophoresis （2-DE）. The 2-DE images were analyzed using software ImageMaster Elite. Some spots with high abundance would express throughout the following specific stages once they were detected. These proteins may play an important role in embryonic development and metabolism. 5 spots of them were excised from the gels and analyzed their sequences and function. These sequences were identified by using matrix-assisted laser desorption ionization- time of flight mass spectrometry （MALDI-TOF-MS）. It is found that five larval functional proteins were expressed in four embryonic stages after homology searching based on peptide mass fingerprinting MS results against related database, that is, juvenile hormone binding protein presented in shortening stage, epidermal growth factor receptor presented in head thorax differentiation stage, larval cuticle protein and accessory gland specific peptide presented in tubercle stage, amylase presented in body pigmentation stage. These functional proteins are seen to express in certain stages for preparing to meet the certain physiological demands, which suggest the larvae relevant genes have been expressed orderly and timely in embryonic stages.

关 键 词：家蚕 胚胎发育 幼虫 基因表达 质谱 肽质量指纹图谱(PMF) 

分 类 号：S882.1[农业科学—特种经济动物饲养] Q71[农业科学—畜牧兽医]