^(60)Co γ射线辐照对东方百合不定芽生长和生理特性影响的初步研究  

The effects of ^(60)Co γ-rays irradiation on growth and physiological characteristics of adventitious bud of oriental lily

作　　者：黄海涛[1] 王丹[1] 周丽娟[1] 赵雪[1] 

机构地区：[1]西南科技大学生命科学与工程学院,绵阳621000

出　　处：《辐射研究与辐射工艺学报》2008年第1期23-27,共5页Journal of Radiation Research and Radiation Processing

基　　金：中国工程物理研究院军民两用开发技术项目(200305)资助

摘　　要：用60Coγ射线以不同辐照剂量(0、0.5、1.0、2.0、4.0、6.0和8.0Gy)对东方百合索邦(Sorbonne)的离体不定芽进行辐照处理,研究辐照对东方百合不定芽生长及生理影响效应。结果表明:随着辐照剂量的增加,东方百合不定芽的丙二醛(Malondialdehyde,MDA)含量、细胞膜渗透性呈上升趋势;辐照剂量≥2.0Gy百合不定芽的超氧阴离子(Superoxide anion,O2-)含量和过氧化氢酶(Catalase,CAT)活性高于对照,出芽率小于对照;辐照剂量≥4.0Gy百合芽高的增量小于对照。说明60Coγ射线辐照对东方百合不定芽的生长有抑制作用。通过综合考虑分析,试验推测60Coγ射线处理东方百合索邦(Sorbonne)组培不定芽的适宜辐照剂量在2.0～4.0Gy之间,其半致死剂量为4.0～6.0Gy。The adventitious buds of orential lily ＂Sorbonne＂ were treated with ^60Coγ-rays at doses of 0, 0.5, 1.0, 2.0, 4.0, 6.0 and 8.0Gy respectively. Effects of ^60Coγ-rays irradiation at different doses on growth and physiological characteristics of adventitious bud of oriental lily have been studied. With the increase of irradiation dose, MDA content and plasmalemma permeability in adventitious bud of oriental lily increases. Superoxide anion （02-） content and catalase （CAT） activity in adventitious bud of oriental lilies which are treated at doses of 2.0, 4.0, 6.0 and 8.0Gy is higher than that in the control group, and germination rates are less than that of the control group. The increment of height of buds which are treated at doses of 4.0, 6.0 and 8.0Gy respectively is less than that of the control group. The results show that ^60Coγ-rays irradiation has the inhibiting effect on the growth of adventitious bud of oriental lily. It can be concluded that the optimum irradiation dose range is from 2.0Gy to 4.0Gy, and LD50 range is from 4.0Gy to 6.0Gy.

关 键 词：^60Coγ射线 东方百合 辐照 不定芽 生长 

分 类 号：Q691.5[生物学—生物物理学] S603.6[农业科学—园艺学]