钙结合蛋白S100A8在放射性肺纤维化早期的表达及意义  

Effect of calcium-binding protein S100A8 expression on early phase of radiation pulmonary fibrosis

作　　者：饶亚岚[1] 李明[1] 丛悦[1] 李峰生[1] 陈肖华[1] 董波[1] 张军权[1] 高玲[1] 毛秉智[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《辐射研究与辐射工艺学报》2008年第1期61-64,共4页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金(30400119)资助

摘　　要：本研究结合体内外实验,探讨了钙结合蛋白S100A8在放射性肺纤维化早期的表达及意义。20Gy60Coγ射线全胸照射大鼠,建立大鼠放射性肺纤维化模型,分别于照射后1周、2周及4周活杀后取肺,采用免疫组化方法检测S100A8蛋白水平的变化;采用RT-PCR方法检测S100A8及与其形成复合物的S100A9 mRNA水平的变化。对于体外培养的小鼠巨噬细胞RAW264.7,采用RT-PCR方法检测该细胞受γ射线与脂多糖(Lipopolysaccharides,LPS)处理后S100A8 mRNA水平的变化。照射后4周,S100A8蛋白在大鼠肺部巨噬细胞胞浆增强表达,S100A8和S100A9 mRNA在大鼠肺部也增强表达。结果表明,RAW264.7细胞中,γ射线与LPS能够协同作用增强该细胞S100A8 mRNA的表达。S100A8在放射性肺纤维化早期于巨噬细胞表达增强,发挥其趋化活性,参与炎症发生,进而在放射性肺纤维化形成过程中发挥作用。The study explores the expression and effect of calcium-binding protein S100A8 on early phase of radiation pulmonary fibrosis via in vivo and in vitro experiments. In vivo experiment, the thoracic regions of rats were irradiated under 20Gy^69Coγ-rays to establish radiation pulmonary fibrosis. After irradiation, the lung specimens of the sacrificed rats were separately harvested by the ends of the first, second, and fourth weeks respectively. The protein expression of S100A8 was tested through immunohistochemistry, the mRNA expression of S100A8 and its heterodimeric S100A9 were investigated by RT-PCR method. In vitro experiment, RT-PCR method was also applied to measure the mRNA expression of S 100A8 in mouse macrophage cell line RAW264.7 after γ-rays irradiation and/or lipopolysaccharide （LPS）. It shows that the protein expression of S100A8 was increased in the plasma of lung macrophages samples and the mRNA expression of S100A8 and S100A9 was also increased in the lung tissue sampies in four weeks after irradiation in vivo experiment. And in vitro experiment it shows that the cooperation between γ-rays and LPS can increase the mRNA expression of S 100A8 in RAW264.7. These phenomena suggest that S 100A8 can exert the chemotactic activity, participate in the inflammatory response, and influence the establishment of radiation pulmonary fibrosis.

关 键 词：放射性肺纤维化 S100A8 巨噬细胞 炎症反应 

分 类 号：Q691.5[生物学—生物物理学] R811.5[医药卫生—放射医学]