杏仁中央核NMDA受体对急性吗啡依赖大鼠戒断诱发条件性位置厌恶的影响  

Effect of NMDA Receptor within the Central Nucleus of the Amygdala on Conditioned Place Aversion Induced by Withdrawal in Acute Morphine-dependent Rats

作　　者：刘彩谊[1] 李勇辉[1] 白云静[1] 隋南[1] 

机构地区：[1]中国科学院心理研究所

出　　处：《中国心理卫生杂志》2008年第2期94-97,共4页Chinese Mental Health Journal

基　　金：国家重点基础研究发展计划(973计划,CB515404);国家自然科学基金重点项目(30230130);中科院“西部之光”人才培养计划项目资助

摘　　要：目的:研究杏仁中央核(Central nucleus of the amygdala,CeA)N-甲基-D-天冬氨酸(N-methyl-D-aspartate,NMDA)受体在纳络酮诱发急性吗啡依赖大鼠戒断性情绪反应中的作用。方法:吗啡、纳络酮间隔4小时皮下注射,通过两轮纳络酮匹配训练建立条件性位置厌恶(Conditioned place aversion,CPA),作为急性吗啡依赖戒断诱发的情绪反应模型。观察纳络酮匹配训练前CeA微量注射NMDA受体拮抗剂地卓西平(MK801)对大鼠训练后在纳络酮匹配侧停留时间的影响。结果:戒断组大鼠经过两轮训练后在纳络酮匹配侧停留时间(230.4±32.8s)比假手术组(457.6±47.8s)显著缩短(P<0.01),训练前CeA微量注射MK801大鼠在纳络酮匹配侧停留时间(447.0±51.8s)比戒断组(230.4±32.8s)显著延长(P<0.01),非戒断组大鼠(急性吗啡依赖组,未接触吗啡组)训练前CeA注射MK801训练前后在处理侧停留时间差异无显著性(P>0.05)。结论:CeA注射MK801既无奖赏效应也无厌恶效应,但能阻断CPA的建立,提示CeA的NMDA受体参与急性吗啡依赖戒断诱发的情绪反应。Objective： To investigate the role of glutamate receptors （NMDA） within the CeA in the negative affective component of morphine withdrawal from acute morphine-dependent rats. Methods： The negative affective component of naloxone-precipitated withdrawal in acute morphine-dependent rats were assessed with the conditioned place aversion paradigm （CPA） . Results： Compared with the sham control group, the rats in withdrawal group spent significantly less time on naloxone-paired compartment （230. 4 ± 32. 8 s/457.6 ± 47. 8 s P 〈0. 01 ） , showing obvious naloxone-induced CPA. But microinjection NMDA receptor antagonist MKS01 into CEA inhibited the development of CPA （447.0 ±51.8s/230. 4 ± 32. 8s, P 〈0. 01） . There was no this effect in blank control or acute dependent group （ those without withdrawal） . Conclusion： The present data suggested that NMDA receptors within the CeA play a key role in the negative affective component of withdrawal from acute dependence on morphine in rats.

关 键 词：吗啡依赖 急性戒断 条件性位置厌恶 杏仁中央核 N-甲基-D-天冬氨酸受体 

分 类 号：R595.5[医药卫生—内科学]