BALB/c及昆明小鼠的汉赛巴尔通体实验室感染模型  

Characterization of infective model in Bartonella henselae-specific immunity in BALB/c and KM mice

作　　者：杨小冉[1] 刘起勇[1] 崔步云[1] 刘小闪[1] 孙继民[1] 林华亮[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所媒介生物控制室

出　　处：《中国媒介生物学及控制杂志》2008年第1期41-43,共3页Chinese Journal of Vector Biology and Control

基　　金：国家自然科学基金资助项目(30371246)

摘　　要：目的用汉赛巴尔通体菌株建立BALB/c和昆明(KM)小鼠的实验室感染模型,检测小鼠体内抗体及菌血症的动态变化。方法用汉赛巴尔通体标准菌株经皮下感染小鼠,用ELISA及PCR检测不同感染天数的小鼠,观察抗体滴度及菌血症的变化。结果BALB/c小鼠在感染后第6天,A值开始升高,第36天达到高峰。KM小鼠的高峰期是在第30天,小鼠在感染的第2天起,血培养出现阳性菌落。结论BALB/c和KM小鼠可作为巴尔通体的实验动物,适于研究巴尔通体在动物体内的动态变化。另外,由于此2种小鼠在感染后持续存在的菌血症,为将来进行动物的巴尔通体细胞免疫和体液免疫反应的研究提供了有价值的证据。Objective Establishing the infective model of BALB/c and KM mice with BartoneUa henselae in the lab, inspecting the dynamic variety of bacteremia and IgG andtibody in the mice. Methods BALB/c and KM mice were inoculated with B. henselae by routes intraperitoneally with a dose of 2.5 × 10^8 cfu/ml, Enzyme-linked immunosorbent assay（ELISA） of serum samples collected as early as 2 d after infection indicated humoral immune responses to B. henselae. Results Specific humoral responses remained through 36 d. From the 6 d PI, the value of A begain to drive up, and the climax was in the 36 d. Culture analysis of blood from mice infected with B. henselae yielded positive results 2 d after infection, and the cultures was detected by polymerase chain reaction by 2 d and up to 36 d. Conclusion Analysis of samples revealed induction of B. henselae-specific immunoglobulin G from 6 to 36 d after infection.

关 键 词：汉赛巴尔通体 BALB/c和昆明小鼠 酶联免疫吸附试验 

分 类 号：R183.7[医药卫生—流行病学]