大花萱草组织培养研究  

Study on tissue culture of Hemerocallis fulva

作　　者：刘志洋[1] 李海涛[2] 朱祥春[2] 孟婧[2] 高继国[2] 

机构地区：[1]哈尔滨市农业科学院,哈尔滨150070 [2]东北农业大学生命科学学院,哈尔滨150030

出　　处：《东北农业大学学报》2008年第1期43-45,共3页Journal of Northeast Agricultural University

基　　金：东北农业大学开放实验室基金

摘　　要：采取大花萱草茎尖为外植体接种在不同培养基上,研究了不同浓度的NAA和6-BA对于外植体生长的影响,并探索了最适于产生愈伤组织和生根的培养基配方。结果表明,有利于外植体产生愈伤组织的培养基配方是MS+6-BA1.0mg·L-1+NAA0.05mg·L-1+30g糖+8g琼脂;适合愈伤组织诱导生芽培养基配方是MS+6-BA1.0mg·L-1+30g糖+8g琼脂;适合幼苗生根的培养基配方是1/2MS+NAA0.5mg·L-1+30g糖+8g琼脂。The stem apex of Hemerocdlis fulva was taken as explant cultured in vitro to induce callus with different culture medium. The effects of different concentration 6-BA and NAA culture medium on explant growth was studied. The results showed that MS＋6-BA 1.0 mg·L^-1＋NAA 0.05 mg·L^-l＋30 g sugar＋8 g agaragar solid culture medium was advantageous to induce callus; after cultured in MS＋6-BA 1.0 mg·L^-1＋30 g sugar＋8 g agaragar, the compact construction callus was formed and differentiation, the clamp seedling was formed; and 1/2 MS＋ NAA 0.5 mg·L^-1＋30g sugar＋8g agaragar solid culture medium was the best medium to induce root.

关 键 词：大花萱草 组织培养 愈伤组织 

分 类 号：S682.19[农业科学—观赏园艺]