慢呼抗口服液中黄芩甙的含量测定  

Content determination of baicalin in Manhukang oral liquid

作　　者：潘晓影[1] 李继昌[1] 柳颖[1] 彭玮[1] 田美湛[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030

出　　处：《东北农业大学学报》2008年第1期104-106,共3页Journal of Northeast Agricultural University

基　　金：黑龙江省博士后科研启动基金(LBH-Q05-26)

摘　　要：采用美国Waters C18(4.6mm×150mm,5μm)色谱柱分离,以甲醇-水-冰醋酸(50:49:1)为流动相,流速为1mL·min-1,检测波长270nm为条件,建立高效液相色谱法测定慢呼抗口服液中黄芩甙的含量。结果表明,此方法线性关系良好(R=0.9995);平均回收率(n=6)99.06%,RSD=1.01%。该法简便、快捷、准确,可以作为慢呼抗口服液质量控制的有效方法。A HPLC method was established for the determination of caffeotannic acid in Manhukang oral liquid. American Waters C18 （4.6 mm×l50 mm, 5 μm） was used, and the mobile phase consisted of colonial spirit-aquaefer-glacial acetic acid （50：49：1 ）, the flow rate was 1.0 mL·min^-1. The detection wave length was 270 nm. The result showed that the linear relationship of this method was well（R=0.9995）, and the average recovery was 99.06% （RSD=1.01%, n=6）. It can be concluded that the HPLC method is simple, rapid and accurate, and can be used for the quality control of Manhukang oral liquid.

关 键 词：慢呼抗口服液 黄苓甙 含量测定 高效液相色谱 

分 类 号：O657.72[理学—分析化学]