^(60)Coγ射线辐照对广藿香离体培养的影响  

THE EFFECT OF ^(60)COγ IRRADIATION ON CULTURE IN VITRO OF Pogostemon cablin(BLANCO) BENTH

作　　者：吴立蓉[1] 贺红[1] 张燕玲[1] 刘星[1] 林小桦[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510405

出　　处：《核农学报》2008年第1期14-17,共4页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金项目(30472152)

摘　　要：研究60Coγ射线辐照对广藿香离体培养的影响,为将辐照诱变技术应用于广藿香新品种选育奠定基础。以广藿香的叶片、带节茎、不带节茎及根为材料进行60Coγ射线辐照试验,采用MT基本培养基,附加0.05mg/L BA进行离体培养。广藿香外植体的死亡率随着辐照剂量的提高而上升,由回归方程导出广藿香叶片、带节茎及不带节茎的辐照半致死剂量分别为72、66和64Gy;外植体再生芽能力,随着辐照剂量的升高而降低。表明辐照会造成外植体的损伤及死亡,对外植体的离体再生有明显的抑制作用,同时,也使部分外植体的再生苗出现一些表型的变化。The ^60Coγ-irradiation on culture in vitro of Pogostemon cablin is studied to breed new variety. The explants used for ^60Coγ-irradiation were the leaf segments, nodular stem segments, stem segments and root tips from Pogostemon cablin. The media for culture in vitro were MT basal media containing 0.05mg/L BA. As irradiation dosage increased, the mortality of different explants from Pogostemon cablin increased. The irradiation dosage of LD50 for leaf segments, nodular stem segments and stem segments were 72,64 and 66Gy, respectively, which were deduced from the regression equation. As the irradiation dosage increased, the ability of the plant regeneration of the explants decreased. ^60Co γ-irradiation could make the explants damage or die. Irradiation could inhibit the regeneration of explants from Pogostemon cablin obviously. The changes in the appearance of the regenerated plants were also observed.

关 键 词：广藿香 ^60Coγ 辐照 外植体 离体培养 

分 类 号：S567.239[农业科学—中草药栽培]